| TAZ-TFM-2015-164 |
Importancia de rotavirus tipo A en conejo de cebo: estudio comparativo entre animales con procesos entéricos y animales sanos.
Domingo Tomás, Paula
Chacón Pérez, Gema (dir.) ; De Blas Giral, Ignacio (dir.)
Universidad de Zaragoza,
VET,
2015
Patología Animal department, Sanidad Animal area
Máster Universitario en Iniciación a la Investigación en Ciencias Veterinarias
Tipo de Trabajo Académico: Trabajo Fin de Master
Notas: Enteric diseases produce serious economic losses in cuniculture due to mortality, decreased growth and worsening conversion rate. The aim of this study was to assess the importance of rotavirus in the differential diagnosis of diarrhoea in fattening rabbits and the relation with other etiological agents. Therefore, digestive tract samples of 90 cases with gastrointestinal symptoms and 40 cases without gastrointestinal symptoms in fattening rabbits between 35 and 55 days of age were analyzed. Samples were microbiologically cultured and PCR real time assay (qPCR) was used for the detection of rotavirus and Escherichia coli eae gene. Eimeria spp. was detected by coprology study and the presence of Clostridium spiroforme and Clostridium perfringens was assessed by Gram strain. Rotavirus was detected in 57.8% of cases of sick animals, nevertheless just 25% of healthy animals were positive (p<0.01). The presence of a single agent did not exceed 2.2%, demonstrating the multifactorial nature of enteric processes. The presence of rotavirus in sick animals increases the presence of other agents: coccidia and enteropathogenic E. coli, p<0.05. To conclude, rotavirus type A is involved in enteric processes in fattening rabbits being necessary to include it in the differential diagnosis.
Notas: Enteric diseases produce serious economic losses in cuniculture due to mortality, decreased growth and worsening conversion rate. The aim of this study was to assess the importance of rotavirus in the differential diagnosis of diarrhoea in fattening rabbits and the relation with other etiological agents. Therefore, digestive tract samples of 90 cases with gastrointestinal symptoms and 40 cases without gastrointestinal symptoms in fattening rabbits between 35 and 55 days of age were analyzed. Samples were microbiologically cultured and PCR real time assay (qPCR) was used for the detection of rotavirus and Escherichia coli eae gene. Eimeria spp. was detected by coprology study and the presence of Clostridium spiroforme and Clostridium perfringens was assessed by Gram strain. Rotavirus was detected in 57.8% of cases of sick animals, nevertheless just 25% of healthy animals were positive (p<0.01). The presence of a single agent did not exceed 2.2%, demonstrating the multifactorial nature of enteric processes. The presence of rotavirus in sick animals increases the presence of other agents: coccidia and enteropathogenic E. coli, p<0.05. To conclude, rotavirus type A is involved in enteric processes in fattening rabbits being necessary to include it in the differential diagnosis.
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