000032169 001__ 32169
000032169 005__ 20210618112019.0
000032169 0247_ $$2doi$$a10.1186/1471-2180-14-21
000032169 0248_ $$2sideral$$a85206
000032169 037__ $$aART-2014-85206
000032169 041__ $$aeng
000032169 100__ $$aCabal, A.
000032169 245__ $$aSingle nucleotide polymorphism (SNP) analysis used for the phylogeny of the Mycobacterium tuberculosis complex based on a pyrosequencing assay
000032169 260__ $$c2014
000032169 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032169 5203_ $$aBackground
Different polymorphisms have been described as markers to classify the lineages of the Mycobacterium tuberculosis complex. The analysis of nine single nucleotide polymorphisms (SNPs) was used to describe seven SNPs cluster groups (SCGs). We attempted to classify those strains that could not been categorized into lineages by the genotyping methods used in the routine testing.
Results
The M. tuberculosis complex isolates collected in 2010 in our region were analysed. A new method based on multiplex-PCRs and pyrosequencing to analyse these SNPs was designed. For the pyrosequencing assay nine SNPs that defined the seven SCGs were selected from the literature: 1977, 74092, 105139, 232574, 311613, 913274, 2460626, 3352929 and gyrA95. In addition, SNPs in katG463, mgtC182, Ag85C103 and RDRio deletion were detected.
000032169 536__ $$9info:eu-repo/grantAgreement/ES/FIS/FIS12/1970$$9info:eu-repo/grantAgreement/ES/FIS/FIS09-051
000032169 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000032169 590__ $$a2.729$$b2014
000032169 591__ $$aMICROBIOLOGY$$b51 / 119 = 0.429$$c2014$$dQ2$$eT2
000032169 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032169 700__ $$aStrunk, M.
000032169 700__ $$aDomínguez, J.
000032169 700__ $$aLezcano, M.A.
000032169 700__ $$0(orcid)0000-0003-4198-6622$$aVitoria, M.A.$$uUniversidad de Zaragoza
000032169 700__ $$aFerrero, M.
000032169 700__ $$aMartín, C.
000032169 700__ $$0(orcid)0000-0003-2266-8602$$aIglesias, M.J.$$uUniversidad de Zaragoza
000032169 700__ $$aSamper, S.
000032169 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000032169 7102_ $$11008$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Medic.Prevent.Salud Públ.
000032169 773__ $$g14, 21 (2014), [9 pp.]$$pBMC Microbiol.$$tBMC MICROBIOLOGY$$x1471-2180
000032169 8564_ $$s934224$$uhttps://zaguan.unizar.es/record/32169/files/texto_completo.pdf$$yVersión publicada
000032169 8564_ $$s106323$$uhttps://zaguan.unizar.es/record/32169/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000032169 909CO $$ooai:zaguan.unizar.es:32169$$particulos$$pdriver
000032169 951__ $$a2021-06-18-11:14:21
000032169 980__ $$aARTICLE