A STD-NMR study of the interaction of the Anabaena Ferredoxin-NAD P+ reductase with the Coenzyme

Antonini, L.V. ; Peregrina, J.R. ; Angulo, J. ; Medina, M. (Universidad de Zaragoza) ; Nieto, P.M.

Resumen: Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope.

Idioma: Inglés

DOI: 10.3390/molecules19010672

Año: 2014

Publicado en: MOLECULES 19, 1 (2014), 672-685
ISSN: 1420-3049

Factor impacto JCR: 2.416 (2014)

Categ. JCR: CHEMISTRY, ORGANIC rank: 22 / 58 = 0.379 (2014) - Q2 - T2

Financiación: info:eu-repo/grantAgreement/ES/MICINN/BIO2010-14983
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CTQ2012-32605

Tipo y forma: Article (Published version)

Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)