A STD-NMR study of the interaction of the Anabaena Ferredoxin-NAD P+ reductase with the Coenzyme
Resumen: Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope.
Idioma: Inglés
DOI: 10.3390/molecules19010672
Año: 2014
Publicado en: MOLECULES 19, 1 (2014), 672-685
ISSN: 1420-3049

Factor impacto JCR: 2.416 (2014)
Categ. JCR: CHEMISTRY, ORGANIC rank: 22 / 58 = 0.379 (2014) - Q2 - T2
Financiación: info:eu-repo/grantAgreement/ES/MICINN/BIO2010-14983
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CTQ2012-32605
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)

Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


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