000032460 001__ 32460
000032460 005__ 20171130135647.0
000032460 0247_ $$2doi$$a10.3390/molecules19010672
000032460 0248_ $$2sideral$$a85181
000032460 037__ $$aART-2014-85181
000032460 041__ $$aeng
000032460 100__ $$aAntonini, L.V.
000032460 245__ $$aA STD-NMR study of the interaction of the Anabaena Ferredoxin-NAD P+ reductase with the Coenzyme
000032460 260__ $$c2014
000032460 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032460 5203_ $$aFerredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope.
000032460 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/BIO2010-14983$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2012-32605
000032460 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000032460 590__ $$a2.416$$b2014
000032460 591__ $$aCHEMISTRY, ORGANIC$$b22 / 58 = 0.379$$c2014$$dQ2$$eT2
000032460 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032460 700__ $$aPeregrina, J.R.
000032460 700__ $$aAngulo, J.
000032460 700__ $$0(orcid)0000-0001-8743-0182$$aMedina, M.$$uUniversidad de Zaragoza
000032460 700__ $$aNieto, P.M.
000032460 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000032460 773__ $$g19, 1 (2014), 672-685$$pMolecules$$tMOLECULES$$x1420-3049
000032460 8564_ $$s702701$$uhttps://zaguan.unizar.es/record/32460/files/texto_completo.pdf$$yVersión publicada
000032460 8564_ $$s84446$$uhttps://zaguan.unizar.es/record/32460/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000032460 909CO $$ooai:zaguan.unizar.es:32460$$particulos$$pdriver
000032460 951__ $$a2017-11-30-13:53:32
000032460 980__ $$aARTICLE