000032489 001__ 32489 000032489 005__ 20170504092419.0 000032489 0247_ $$2doi$$a10.1186/1756-3305-7-223 000032489 0248_ $$2sideral$$a92395 000032489 037__ $$aART-2014-92395 000032489 041__ $$aeng 000032489 100__ $$aGarros, C. 000032489 245__ $$aTowards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): Results from an international ring trial targeting four species of the subgenus Avaritia 000032489 260__ $$c2014 000032489 5060_ $$aAccess copy available to the general public$$fUnrestricted 000032489 5203_ $$aBackground: Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly. Methods. In this study, designed as an inter-laboratory ring trial with twelve partners from Europe and North Africa, we assess four PCR-based assays which are used routinely to differentiate the four species of Culicoides listed above. The assays based on mitochondrial or ribosomal DNA or microarray hybridisation were tested using aliquots of Culicoides DNA (extracted using commercial kits), crude lysates of ground specimens and whole Culicoides (265 individuals), and non-Culicoides Ceratopogonidae (13 individuals) collected from across Europe. Results: A total of 800 molecular assays were implemented. The in-house assays functioned effectively, although specificity and sensitivity varied according to the molecular marker and DNA extraction method used. The Obsoletus group specificity was overall high (95-99%) while the sensitivity varied greatly (59.6-100%). DNA extraction methods impacted the sensitivity of the assays as well as the type of sample used as template for the DNA extraction. Conclusions: The results are discussed in terms of current use of species diagnostic assays and the future development of molecular tools for the rapid differentiation of cryptic Culicoides species. 000032489 536__ $$9info:eu-repo/grantAgreement/EUR/FP6/SSPE-CT-2006-044285 000032489 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000032489 590__ $$a3.43$$b2014 000032489 591__ $$aPARASITOLOGY$$b7 / 36 = 0.194$$c2014$$dQ1$$eT1 000032489 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000032489 700__ $$aBalenghien, T. 000032489 700__ $$aCarpenter, S. 000032489 700__ $$aDelécolle, J.C 000032489 700__ $$aMeiswinkel, R. 000032489 700__ $$aPédarrieu, A. 000032489 700__ $$aRakotoarivony, I. 000032489 700__ $$aGardès, L. 000032489 700__ $$aGolding, N. 000032489 700__ $$aBarber, J. 000032489 700__ $$aMiranda, M. 000032489 700__ $$aBorràs, D.B. 000032489 700__ $$aGoffredo, M. 000032489 700__ $$aMonaco, F. 000032489 700__ $$aPagès, N. 000032489 700__ $$aSghaier, S. 000032489 700__ $$aHammami, S. 000032489 700__ $$0(orcid)0000-0001-9513-0219$$aCalvo, J.H. 000032489 700__ $$0(orcid)0000-0003-0663-8411$$aLucientes, J.$$uUniversidad de Zaragoza 000032489 700__ $$aGeysen, D. 000032489 700__ $$aDe Deken, G. 000032489 700__ $$aSarto, I.Monteys 000032489 700__ $$aSchwenkenbecher, J. 000032489 700__ $$aKampen, H. 000032489 700__ $$aHoffmann, B. 000032489 700__ $$aLehmann, K. 000032489 700__ $$aWerner, D. 000032489 700__ $$aBaldet, T. 000032489 700__ $$aLancelot, R. 000032489 700__ $$aCêtre-Sossah, C. 000032489 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal 000032489 773__ $$g7 (2014), 233 [9 pp.]$$pParasites & Vectors$$tParasites & Vectors$$x1756-3305 000032489 8564_ $$s249332$$uhttps://zaguan.unizar.es/record/32489/files/texto_completo.pdf$$yVersión publicada 000032489 8564_ $$s82814$$uhttps://zaguan.unizar.es/record/32489/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000032489 909CO $$ooai:zaguan.unizar.es:32489$$particulos$$pdriver 000032489 951__ $$a2017-05-04-09:18:50 000032489 980__ $$aARTICLE