Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints

Ardanaz, Nekane ; Vázquez, Francisco José (Universidad de Zaragoza) ; Romero, Antonio (Universidad de Zaragoza) ; Remacha, Ana Rosa (Universidad de Zaragoza) ; Barrachina, Laura (Universidad de Zaragoza) ; Sanz, Arianne (Universidad de Zaragoza) ; Ranera, Beatriz ; Vitoria, Arantza (Universidad de Zaragoza) ; Albareda, Jorge (Universidad de Zaragoza) ; Prades, Marta ; Zaragoza, Pilar (Universidad de Zaragoza) ; Martín-Burriel, Inmaculada (Universidad de Zaragoza) ; Rodellar, Clementina (Universidad de Zaragoza)

Resumen: Mesenchymal stem cells (MSCs) transplantation has become a promising therapeutic choice for musculoskeletal injuries. Joint-related disorders are highly prevalent in horses. Therefore, these animals are considered as suitable models for testing MSC-based therapies for these diseases. The aim of this study was to investigate the clinical and inflammatory responses to intra-articular single and repeat dose administration of autologous or of pooled allogeneic MSCs in healthy equine healthy joints. Six horses were intra-articularly injected with a single autologous dose of bone marrow derived MSCs (BM-MSCs) and two separate doses of allogeneic BM-MSCs pooled from several donors. All contralateral joints were injected with Lactated Ringer’s Solution (LRS) as the control vehicle. Signs of synovitis and lameness were evaluated at days 0, 1, 2, 3, 5 and 10 after injection. Total protein (TP), white blood cell count (WBC) and neutrophil count (NC) in synovial fluid were also measured at the same time-points.

Idioma: Inglés

DOI: 10.1186/s12917-016-0692-x

Año: 2016

Publicado en: BMC VETERINARY RESEARCH 12, 1 (2016), 65 [9 pp.]
ISSN: 1746-6148

Factor impacto JCR: 1.75 (2016)

Categ. JCR: VETERINARY SCIENCES rank: 24 / 136 = 0.176 (2016) - Q1 - T1

Factor impacto SCIMAGO: 0.87 - Veterinary (miscellaneous) (Q1) - Medicine (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/MICINN/AGL2011-28609

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Traumatología y Ortopedia (Dpto. Cirugía,Ginecol.Obstetr.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)