Individual constituents from essential oils inhibit biofilm mass production by multi-drug resistant staphylococcus aureus

Espina, L. ; Pagán, R. (Universidad de Zaragoza) ; López, D. ; García-Gonzalo, D. (Universidad de Zaragoza)

Resumen: Biofilm formation by Staphylococcus aureus represents a problem in both the medical field and the food industry, because the biofilm structure provides protection to embedded cells and it strongly attaches to surfaces. This circumstance is leading to many research programs seeking new alternatives to control biofilm formation by this pathogen. In this study we show that a potent inhibition of biofilm mass production can be achieved in community-associated methicillin-resistant S. aureus (CA-MRSA) and methicillin-sensitive strains using plant compounds, such as individual constituents (ICs) of essential oils (carvacrol, citral, and (+)-limonene). The Crystal Violet staining technique was used to evaluate biofilm mass formation during 40 h of incubation. Carvacrol is the most effective IC, abrogating biofilm formation in all strains tested, while CA-MRSA was the most sensitive phenotype to any of the ICs tested. Inhibition of planktonic cells by ICs during initial growth stages could partially explain the inhibition of biofilm formation. Overall, our results show the potential of EOs to prevent biofilm formation, especially in strains that exhibit resistance to other antimicrobials. As these compounds are food additives generally recognized as safe, their anti-biofilm properties may lead to important new applications, such as sanitizers, in the food industry or in clinical settings.

Idioma: Inglés

DOI: 10.3390/molecules200611357

Año: 2015

Publicado en: Molecules 20, 6 (2015), 11357-11372
ISSN: 1420-3049

Factor impacto JCR: 2.465 (2015)

Categ. JCR: CHEMISTRY, ORGANIC rank: 24 / 59 = 0.407 (2015) - Q2 - T2

Factor impacto SCIMAGO: 0.57 - Analytical Chemistry (Q2) - Chemistry (miscellaneous) (Q2) - Drug Discovery (Q2) - Physical and Theoretical Chemistry (Q2) - Medicine (miscellaneous) (Q2) - Organic Chemistry (Q2) - Pharmaceutical Science (Q2) - Molecular Medicine (Q3)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)