Chitosan feasibility to retain retinal stem cell phenotype and slow proliferation for retinal transplantation

Srivastava, Girish Kumar ; Rodríguez-Crespo, David ; Singh, Amar Kumar ; Casado-Coterillo, Clara ; Fernandez-Bueno, Ivan ; Garcia-Gutierrez, Maria T. ; Coronas, Joaquin (Universidad de Zaragoza) ; Pastor, J. Carlos

Resumen: Retinal stem cells (RSCs) are promising in cell replacement strategies for retinal diseases. RSCs can migrate, differentiate, and integrate into retina. However, RSCs transplantation needs an adequate support; chitosan membrane (ChM) could be one, which can carry RSCs with high feasibility to support their integration into retina. RSCs were isolated, evaluated for phenotype, and subsequently grown on sterilized ChM and polystyrene surface for 8 hours, 1, 4, and 11 days for analysing cell adhesion, proliferation, viability, and phenotype. Isolated RSCs expressed GFAP, PKC, isolectin, recoverin, RPE65, PAX-6, cytokeratin 8/18, and nestin proteins. They adhered (28 ± 16%, 8 hours) and proliferated (40 ± 20 cells/field, day 1 and 244 ± 100 cells/field, day 4) significantly low on ChM. However, they maintained similar viability (>95%) and phenotype (cytokeratin 8/18, PAX6, and nestin proteins expression, day 11) on both surfaces (ChM and polystyrene). RSCs did not express alpha-SMA protein on both surfaces. RSCs express proteins belonging to epithelial, glial, and neural cells, confirming that they need further stimulus to reach a final destination of differentiation that could be provided in in vivo condition. ChM does not alternate RSCs behaviour and therefore can be used as a cell carrier so that slow proliferating RSCs can migrate and integrate into retina.

Idioma: Inglés

DOI: 10.1155/2014/287896

Año: 2014

Publicado en: BioMed Research International 2014 (2014), 287896 [10 pp]
ISSN: 2314-6133

Factor impacto JCR: 1.579 (2014)

Categ. JCR: MEDICINE, RESEARCH & EXPERIMENTAL rank: 85 / 123 = 0.691 (2014) - Q3 - T3
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 106 / 162 = 0.654 (2014) - Q3 - T2

Financiación: info:eu-repo/grantAgreement/ES/MICINN/ISCIII-PS09-00938

Tipo y forma: Article (Published version)

Área (Departamento): Ingeniería Química (Departamento de Ingeniería Química y Tecnologías del Medio Ambiente)