Sex differences in constitutive autophagy

Oliván, S. (Universidad de Zaragoza) ; Calvo, A.C. (Universidad de Zaragoza) ; Manzano, R. ; Zaragoza, P. (Universidad de Zaragoza) ; Osta, R. (Universidad de Zaragoza)

Resumen: Sex bias has been described nowadays in biomedical research on animal models, although sexual dimorphism has been confirmed widely under pathological and physiological conditions. The main objective of our work was to study the sex differences in constitutive autophagy in spinal cord and skeletalmuscle tissue fromwild type mice. To examine the influence of sex on autophagy, mRNA and proteins were extracted from male and female mice tissues.The expressions of microtubule-associated protein 1 light chain 3 (LC3) and sequestosome 1 (p62), markers to monitor autophagy, were analyzed at 40, 60, 90, and 120 days of age.We found significant sex differences in the expression of LC3 and p62 in both tissues at these ages. The results indicated that sex and tissue specific differences exist in constitutive autophagy.These data underlined the need to include both sexes in the experimental groups to minimize any sex bias.

Idioma: Inglés

DOI: 10.1155/2014/652817

Año: 2014

Publicado en: BioMed Research International 2014 (2014), 652817 [5 pp]
ISSN: 2314-6133

Factor impacto JCR: 1.579 (2014)

Categ. JCR: MEDICINE, RESEARCH & EXPERIMENTAL rank: 85 / 123 = 0.691 (2014) - Q3 - T3
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 107 / 163 = 0.656 (2014) - Q3 - T2

Financiación: info:eu-repo/grantAgreement/ES/FIS/PI10-0178
Financiación: info:eu-repo/grantAgreement/ES/MICINN/IPT-2011–1091-900000

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)