Fluorescence of the Flavin group in choline oxidase. Insights and analytical applications for the determination of choline and betaine aldehyde
Ortega, E. ; De Marcos, S. (Universidad de Zaragoza) ; Sanz-Vicente, I. (Universidad de Zaragoza) ; Ubide, C. ; Ostra, M. ; Vidal, M. ; Galbán, J. (Universidad de Zaragoza)
Resumen: Choline oxidase (ChOx) is a flavoenzyme catalysing the oxidation of choline (Ch) to betaine aldehyde (BA) and glycine betaine (GB). In this paper a fundamental study of the intrinsic fluorescence properties of ChOx due to Flavin Adenine Dinucleotide (FAD) is presented and some analytical applications are studied in detail. Firstly, an unusual alteration in the excitation spectra, in comparison with the absorption spectra, has been observed as a function of the pH. This is ascribed to a change of polarity in the excited state. Secondly, the evolution of the fluorescence spectra during the reaction seems to indicate that the reaction takes place in two consecutive, but partially overlapped, steps and each of them follows a different mechanism. Thirdly, the chemical system can be used to determine the Ch concentration in the range from 5×10-6 M to 5×10-5 M (univariate and multivariate calibration) in the presence of BA as interference, and the joint Ch+BA concentration in the range 5×10-6-5×10-4 M (multivariate calibration) with mean errors under 10%//a semiquantitative determination of the BA concentration can be deduced by difference. Finally, Ch has been successfully determined in an infant milk sample.
Idioma: Inglés
DOI: 10.1016/j.talanta.2015.09.060
Año: 2016
Publicado en: TALANTA 147 (2016), 253-260
ISSN: 0039-9140
Factor impacto JCR: 4.162 (2016)
Categ. JCR: CHEMISTRY, ANALYTICAL rank: 9 / 76 = 0.118 (2016) - Q1 - T1
Factor impacto SCIMAGO: 1.167 - Chemistry (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA/E74
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material.
Exportado de SIDERAL (2020-02-21-13:22:34)
Visitas y descargas
Idioma: Inglés
DOI: 10.1016/j.talanta.2015.09.060
Año: 2016
Publicado en: TALANTA 147 (2016), 253-260
ISSN: 0039-9140
Factor impacto JCR: 4.162 (2016)
Categ. JCR: CHEMISTRY, ANALYTICAL rank: 9 / 76 = 0.118 (2016) - Q1 - T1
Factor impacto SCIMAGO: 1.167 - Chemistry (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA/E74
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. Exportado de SIDERAL (2020-02-21-13:22:34)
Permalink:
Visitas y descargas
Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Química Analítica
Record created 2017-03-13, last modified 2020-02-21