A rabbit model of Acanthamoeba keratitis: Use of infected soft contact lenses after corneal epithelium debridement with a diamond burr

Ortillés, Á. (Universidad de Zaragoza) ; Goñi, P. (Universidad de Zaragoza) ; Rubio, E. (Universidad de Zaragoza) ; Sierra, M. (Universidad de Zaragoza) ; Gámez, E. ; Fernández, M.T. (Universidad de Zaragoza) ; Benito, M. (Universidad de Zaragoza) ; Cristóbal, J.Á. ; Calvo, B. (Universidad de Zaragoza)

Resumen: PURPOSE. To develop a rabbit model of Acanthamoeba keratitis (AK) as the best method to reproduce the natural course of this disease. METHODS. To induce AK, infected contact lenses (1000 amoebae/mm2, 90% trophozoites) were placed over the previously debrided corneal surface, in combination with a temporary tarsorrhaphy. Environmental and clinical strains of Acanthamoeba spp. (genotype T4) were used. Three groups (1L, n = 32; 2L–21d, n = 5; 2L–3d, n = 23) were established according to the number of contact lenses used (1L, 1 lens; 2L–21d and 2L–3d, 2 lenses) and the placement day of these (1L, day 1; 2L–21d, days 1 and 21; 2L–3d, days 1 and 3). The infection was quantified by a clinical score system and confirmed using corneal cytology and culture, polymerase chain reaction and histopathologic analysis. RESULTS. The infection rate obtained was high (1L, 87.5%; 2L–21d, 100%; 2L–3d, 82.6%), although no clinical signs were observed in the 50% of the infected animals in group 1L. Among groups, group 2L–3d showed more cases of moderate and severe infection. Among strains, no statistically significant differences were found in the infection rate. In the control eyes, cross infection was confirmed when a sterile contact lens was placed in the previously debrided corneas but not if the eye remained intact. CONCLUSIONS. The combination of two infected contact lenses after corneal debridement seems to be an alternative model, clinically and histopathologically similar to its human counterpart, to induce the different AK stages and reproduce the course of the disease in rabbits.

Idioma: Inglés

DOI: 10.1167/iovs.16-21100

Año: 2017

Publicado en: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 58, 2 (2017), 1218-1227
ISSN: 0146-0404

Factor impacto JCR: 3.388 (2017)

Categ. JCR: OPHTHALMOLOGY rank: 9 / 59 = 0.153 (2017) - Q1 - T1

Factor impacto SCIMAGO: 2.058 - Cellular and Molecular Neuroscience (Q1) - Sensory Systems (Q1) - Ophthalmology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B124
Financiación: info:eu-repo/grantAgreement/ES/DGA/T88
Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU13-03782
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Parasitología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Enfermería (Dpto. Fisiatría y Enfermería)
Área (Departamento): Área Farmacología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Medic.Prevent.Salud Públ. (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)