Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs
Mainar-Jaime, R. (Universidad de Zaragoza) ; Andrés, S. ; Vico, J.P. ; San Román, B. ; Garrido, V. ; Grilló, M.J.
Resumen: The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations with different Salmonella prevalences (14 farms with a =20% prevalence and 13 farms with a <20% prevalence) and through the use of latent-class models in concert with Bayesian inference, assuming 100% ISO specificity, and an invA-based PCR as the second diagnostic method. The SeISO was estimated to be close to 87%, while the sensitivity of the PCR reached up to 83.6% and its specificity was 97.4%. Interestingly, the bacteriological reanalysis of 33 potential false-negative (PCR-positive) samples allowed isolation of 19 (57.5%) new Salmonella strains, improving the overall diagnostic accuracy of the bacteriology. Considering the usual limitations of bacteriology regarding Se, these results support the adequacy of the ISO for the detection of Salmonella spp. from MLN and also that of the PCR-based method as an alternative or complementary (screening) test for the diagnosis of pig salmonellosis, particularly considering the cost and time benefits of the molecular procedure.
Idioma: Inglés
DOI: 10.1128/JCM.02099-12
Año: 2013
Publicado en: JOURNAL OF CLINICAL MICROBIOLOGY 51, 1 (2013), 89-94
ISSN: 0095-1137
Factor impacto JCR: 4.232 (2013)
Categ. JCR: MICROBIOLOGY rank: 23 / 118 = 0.195 (2013) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/INIA/FAU2008-16
Financiación: info:eu-repo/grantAgreement/ES/INIA/RTA2007-65
Tipo y forma: Article (Published version)
Área (Departamento): Sanidad Animal (Departamento de Patología Animal)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Idioma: Inglés
DOI: 10.1128/JCM.02099-12
Año: 2013
Publicado en: JOURNAL OF CLINICAL MICROBIOLOGY 51, 1 (2013), 89-94
ISSN: 0095-1137
Factor impacto JCR: 4.232 (2013)
Categ. JCR: MICROBIOLOGY rank: 23 / 118 = 0.195 (2013) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/INIA/FAU2008-16
Financiación: info:eu-repo/grantAgreement/ES/INIA/RTA2007-65
Tipo y forma: Article (Published version)
Área (Departamento): Sanidad Animal (Departamento de Patología Animal)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2017-05-03-14:22:46)
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Record created 2017-03-29, last modified 2017-05-03