Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie

Garza, M.C. (Universidad de Zaragoza) ; Fernández-Borges, N. ; Bolea, R. (Universidad de Zaragoza) ; Badiola, J.J. (Universidad de Zaragoza) ; Castilla, J. ; Monleón, E. (Universidad de Zaragoza)
Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
Resumen: Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
Idioma: Inglés
DOI: 10.1371/journal.pone.0027525
Año: 2011
Publicado en: PLoS ONE 6, 12 (2011), e27525 [5 pp]
ISSN: 1932-6203

Factor impacto JCR: 4.092 (2011)
Categ. JCR: BIOLOGY rank: 12 / 85 = 0.141 (2011) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MEC/AP2007-03842
Financiación: info:eu-repo/grantAgreement/ES/MICINN/AGL2009-11553-C02-01
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)


Creative Commons Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.


Exportado de SIDERAL (2022-02-08-11:23:30)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Artículos



 Registro creado el 2017-05-19, última modificación el 2022-02-08


Versión publicada:
 PDF
Valore este documento:

Rate this document:
1
2
3
 
(Sin ninguna reseña)