000061331 001__ 61331
000061331 005__ 20220208112844.0
000061331 0247_ $$2doi$$a10.1371/journal.pone.0027525
000061331 0248_ $$2sideral$$a75890
000061331 037__ $$aART-2011-75890
000061331 041__ $$aeng
000061331 100__ $$0(orcid)0000-0001-7098-2327$$aGarza, M.C.$$uUniversidad de Zaragoza
000061331 245__ $$aDetection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
000061331 260__ $$c2011
000061331 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061331 5203_ $$aScrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
000061331 536__ $$9info:eu-repo/grantAgreement/ES/MEC/AP2007-03842$$9info:eu-repo/grantAgreement/ES/MICINN/AGL2009-11553-C02-01
000061331 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061331 590__ $$a4.092$$b2011
000061331 591__ $$aBIOLOGY$$b12 / 85 = 0.141$$c2011$$dQ1$$eT1
000061331 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061331 700__ $$aFernández-Borges, N.
000061331 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R.$$uUniversidad de Zaragoza
000061331 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000061331 700__ $$aCastilla, J.
000061331 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, E.$$uUniversidad de Zaragoza
000061331 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000061331 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000061331 773__ $$g6, 12 (2011), e27525 [5 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203
000061331 8564_ $$s381354$$uhttps://zaguan.unizar.es/record/61331/files/texto_completo.pdf$$yVersión publicada
000061331 8564_ $$s123961$$uhttps://zaguan.unizar.es/record/61331/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061331 909CO $$ooai:zaguan.unizar.es:61331$$particulos$$pdriver
000061331 951__ $$a2022-02-08-11:23:30
000061331 980__ $$aARTICLE