Transcriptomic profiling of TK2 deficient human skeletal muscle suggests a role for the p53 signalling pathway and identifies growth and differentiation factor-15 as a potential novel biomarker for mitochondrial myopathies
Kalko, S.G. ; Paco, S. ; Jou, C. ; Rodríguez, M.A. ; Meznaric, M. ; Rogac, M. ; Jekovec-Vrhovsek, M. ; Sciacco, M. ; Moggio, M. ; Fagiolari, G. ; De Paepe, B. ; De Meirleir, L. ; Ferrer, I. ; Roig-Quilis, M. ; Munell, F. ; Montoya, J. (Universidad de Zaragoza) ; López-Gallardo, E. ; Ruiz-Pesini, E. (Universidad de Zaragoza) ; Artuch, R. ; Montero, R. ; Torner, F. ; Nascimento, A. ; Ortez, C. ; Colomer, J. ; Jimenez-Mallebrera, C.
Resumen: Background
Mutations in the gene encoding thymidine kinase 2 (TK2) result in the myopathic form of mitochondrial DNA depletion syndrome which is a mitochondrial encephalomyopathy presenting in children. In order to unveil some of the mechanisms involved in this pathology and to identify potential biomarkers and therapeutic targets we have investigated the gene expression profile of human skeletal muscle deficient for TK2 using cDNA microarrays.
Results
We have analysed the whole transcriptome of skeletal muscle from patients with TK2 mutations and compared it to normal muscle and to muscle from patients with other mitochondrial myopathies. We have identified a set of over 700 genes which are differentially expressed in TK2 deficient muscle. Bioinformatics analysis reveals important changes in muscle metabolism, in particular, in glucose and glycogen utilisation, and activation of the starvation response which affects aminoacid and lipid metabolism. We have identified those transcriptional regulators which are likely to be responsible for the observed changes in gene expression.
Conclusion
Our data point towards the tumor suppressor p53 as the regulator at the centre of a network of genes which are responsible for a coordinated response to TK2 mutations which involves inflammation, activation of muscle cell death by apoptosis and induction of growth and differentiation factor 15 (GDF-15) in muscle and serum. We propose that GDF-15 may represent a potential novel biomarker for mitochondrial dysfunction although further studies are required.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-91
Año: 2014
Publicado en: BMC Genomics 15, 1 (2014), 91 [22 PP]
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA/B33
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI10-00662
Financiación: info:eu-repo/grantAgreement/ES/MICINN/ISCIII-CP09-00011
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.
Exportado de SIDERAL (2017-06-12-09:14:23)
Visitas y descargas
Mutations in the gene encoding thymidine kinase 2 (TK2) result in the myopathic form of mitochondrial DNA depletion syndrome which is a mitochondrial encephalomyopathy presenting in children. In order to unveil some of the mechanisms involved in this pathology and to identify potential biomarkers and therapeutic targets we have investigated the gene expression profile of human skeletal muscle deficient for TK2 using cDNA microarrays.
Results
We have analysed the whole transcriptome of skeletal muscle from patients with TK2 mutations and compared it to normal muscle and to muscle from patients with other mitochondrial myopathies. We have identified a set of over 700 genes which are differentially expressed in TK2 deficient muscle. Bioinformatics analysis reveals important changes in muscle metabolism, in particular, in glucose and glycogen utilisation, and activation of the starvation response which affects aminoacid and lipid metabolism. We have identified those transcriptional regulators which are likely to be responsible for the observed changes in gene expression.
Conclusion
Our data point towards the tumor suppressor p53 as the regulator at the centre of a network of genes which are responsible for a coordinated response to TK2 mutations which involves inflammation, activation of muscle cell death by apoptosis and induction of growth and differentiation factor 15 (GDF-15) in muscle and serum. We propose that GDF-15 may represent a potential novel biomarker for mitochondrial dysfunction although further studies are required.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-91
Año: 2014
Publicado en: BMC Genomics 15, 1 (2014), 91 [22 PP]
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA/B33
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI10-00662
Financiación: info:eu-repo/grantAgreement/ES/MICINN/ISCIII-CP09-00011
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. Exportado de SIDERAL (2017-06-12-09:14:23)
Enlace permanente:
Visitas y descargas
Este artículo se encuentra en las siguientes colecciones:
Artículos > Artículos por área > Bioquímica y Biología Molecular
Registro creado el 2017-06-12, última modificación el 2017-06-12