000061616 001__ 61616 000061616 005__ 20190709135445.0 000061616 0247_ $$2doi$$a10.1038/s41598-017-00949-4 000061616 0248_ $$2sideral$$a98931 000061616 037__ $$aART-2017-98931 000061616 041__ $$aeng 000061616 100__ $$aAhmed, M. 000061616 245__ $$aAgarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines /631/1647/767/70 /631/154/1435 /123 /13/21 /14/63 /13/31 article 000061616 260__ $$c2017 000061616 5060_ $$aAccess copy available to the general public$$fUnrestricted 000061616 5203_ $$aWe describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in which cells are suspended. As chemokine molecules diffuse away from the spot, a transient chemoattractant gradient is established across the spots. Cells expressing the corresponding cognate chemokine receptors migrate against this gradient by crawling under the agarose spots towards their centre. We show that this migration is chemokine-specific; meaning that only cells that express the cognate chemokine cell surface receptor, migrate under the spot containing its corresponding chemokine ligand. Furthermore, we show that migration under the agarose spot can be modulated by selective small molecule antagonists present in the cell culture medium. 000061616 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000061616 590__ $$a4.122$$b2017 000061616 591__ $$aMULTIDISCIPLINARY SCIENCES$$b12 / 64 = 0.188$$c2017$$dQ1$$eT1 000061616 592__ $$a1.533$$b2017 000061616 593__ $$aMultidisciplinary$$c2017$$dQ1 000061616 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000061616 700__ $$aBasheer, H.A. 000061616 700__ $$0(orcid)0000-0002-9414-1845$$aAyuso, J.M. 000061616 700__ $$aAhmet, D. 000061616 700__ $$aMazzini, M. 000061616 700__ $$aPatel, R. 000061616 700__ $$aShnyder, S.D. 000061616 700__ $$aVinader, V. 000061616 700__ $$aAfarinkia, K. 000061616 773__ $$g7, 1 (2017), 1075 [11 pp]$$pSci. rep.$$tScientific reports$$x2045-2322 000061616 8564_ $$s4935968$$uhttps://zaguan.unizar.es/record/61616/files/texto_completo.pdf$$yVersión publicada 000061616 8564_ $$s111997$$uhttps://zaguan.unizar.es/record/61616/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000061616 909CO $$ooai:zaguan.unizar.es:61616$$particulos$$pdriver 000061616 951__ $$a2019-07-09-11:38:05 000061616 980__ $$aARTICLE