000061712 001__ 61712
000061712 005__ 20190709135446.0
000061712 0247_ $$2doi$$a10.18632/oncotarget.13693
000061712 0248_ $$2sideral$$a99206
000061712 037__ $$aART-2017-99206
000061712 041__ $$aeng
000061712 100__ $$aRabjerg, M.
000061712 245__ $$aNuclear localization of the CK2a-subunit correlates with poor prognosis in clear cell renal cell carcinoma
000061712 260__ $$c2017
000061712 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061712 5203_ $$aProtein kinase CK2a, one of the two catalytic isoforms of the protein kinase CK2 has been shown to contribute to tumor development, tumor proliferation and suppression of apoptosis in various malignancies. We conducted this study to investigate CK2 expression in different subtypes of Renal Cell Carcinoma (RCC) and in the benign oncocytoma. qRT-PCR, immunohistochemistry and Western blot analyses revealed that CK2a expression was significantly increased at the mRNA and protein levels in clear cell RCC (ccRCC). Also the kinase activity of CK2 was significantly increased in ccRCC compared to normal renal cortex. Nuclear protein expression of CK2a correlated in univariate analysis with poor Progression Free Survival (HR = 8.11, p = 0.016). Functional analyses (cell proliferation assay) revealed an inhibitory effect of Caki-2 cell growth following CK2 inhibition with CX-4945. Our results suggest that CK2a promotes migration and invasion of ccRCC and therefore could serve as a novel prognostic biomarker and molecular therapeutic target in this type of cancer.
000061712 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061712 592__ $$a1.942$$b2017
000061712 593__ $$aOncology$$c2017$$dQ1
000061712 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061712 700__ $$aGuerra, B.
000061712 700__ $$0(orcid)0000-0001-5348-924X$$aOliván-Viguera, A.
000061712 700__ $$aMikkelsen, M.L.N.
000061712 700__ $$aKöhler, R.
000061712 700__ $$aIssinger, O.G.
000061712 700__ $$aMarcussen, N.
000061712 773__ $$g8, 1 (2017), 1613-1627$$pONCOTARGET$$tOncotarget$$x1949-2553
000061712 8564_ $$s5012914$$uhttps://zaguan.unizar.es/record/61712/files/texto_completo.pdf$$yVersión publicada
000061712 8564_ $$s120454$$uhttps://zaguan.unizar.es/record/61712/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061712 909CO $$ooai:zaguan.unizar.es:61712$$particulos$$pdriver
000061712 951__ $$a2019-07-09-11:38:30
000061712 980__ $$aARTICLE