Gene expression profiling of mesenteric lymph nodes from sheep with natural scrapie
Filali, H. ; Martín-Burriel, I. (Universidad de Zaragoza) ; Harders, F. ; Varona, L. (Universidad de Zaragoza) ; Hedman, C. (Universidad de Zaragoza) ; Mediano, D.R. ; Monzón, M. (Universidad de Zaragoza) ; Bossers, A. ; Badiola, J.J. (Universidad de Zaragoza) ; Bolea, R. (Universidad de Zaragoza)
Resumen: Background:
Prion diseases are characterized by the accumulation of the pathogenic PrPSc protein, mainly in the brain and the lymphoreticular system. Although prions multiply/accumulate in the lymph nodes without any detectable pathology, transcriptional changes in this tissue may reflect biological processes that contribute to the molecular pathogenesis of prion diseases. Little is known about the molecular processes that occur in the lymphoreticular system in early and late stages of prion disease. We performed a microarray-based study to identify genes that are differentially expressed at different disease stages in the mesenteric lymph node of sheep naturally infected with scrapie. Oligo DNA microarrays were used to identify gene-expression profiles in the early/middle (preclinical) and late (clinical) stages of the disease.
Results:
In the clinical stage of the disease, we detected 105 genes that were differentially expressed (=2-fold change in expression). Of these, 43 were upregulated and 62 downregulated as compared with age-matched negative controls. Fewer genes (50) were differentially expressed in the preclinical stage of the disease. Gene Ontology enrichment analysis revealed that the differentially expressed genes were largely associated with the following terms: glycoprotein, extracellular region, disulfide bond, cell cycle and extracellular matrix. Moreover, some of the annotated genes could be grouped into 3 specific signaling pathways: focal adhesion, PPAR signaling and ECM-receptor interaction. We discuss the relationship between the observed gene expression profiles and PrPSc deposition and the potential involvement in the pathogenesis of scrapie of 7 specific differentially expressed genes whose expression levels were confirmed by real time-PCR.
Conclusions:
The present findings identify new genes that may be involved in the pathogenesis of natural scrapie infection in the lymphoreticular system, and confirm previous reports describing scrapie-induced alterations in the expression of genes involved in protein misfolding, angiogenesis and the oxidative stress response. Further studies will be necessary to determine the role of these genes in prion replication, dissemination and in the response of the organism to this disease.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-59
Año: 2014
Publicado en: BMC Genomics 15, 59 (2014), 1-17
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2008-0256
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Sanidad Animal (Departamento de Patología Animal)
Área (Departamento): Genética (Departamento de Anatomía, Embriología y Genética Animal)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.
Exportado de SIDERAL (2017-12-11-08:30:08)
Prion diseases are characterized by the accumulation of the pathogenic PrPSc protein, mainly in the brain and the lymphoreticular system. Although prions multiply/accumulate in the lymph nodes without any detectable pathology, transcriptional changes in this tissue may reflect biological processes that contribute to the molecular pathogenesis of prion diseases. Little is known about the molecular processes that occur in the lymphoreticular system in early and late stages of prion disease. We performed a microarray-based study to identify genes that are differentially expressed at different disease stages in the mesenteric lymph node of sheep naturally infected with scrapie. Oligo DNA microarrays were used to identify gene-expression profiles in the early/middle (preclinical) and late (clinical) stages of the disease.
Results:
In the clinical stage of the disease, we detected 105 genes that were differentially expressed (=2-fold change in expression). Of these, 43 were upregulated and 62 downregulated as compared with age-matched negative controls. Fewer genes (50) were differentially expressed in the preclinical stage of the disease. Gene Ontology enrichment analysis revealed that the differentially expressed genes were largely associated with the following terms: glycoprotein, extracellular region, disulfide bond, cell cycle and extracellular matrix. Moreover, some of the annotated genes could be grouped into 3 specific signaling pathways: focal adhesion, PPAR signaling and ECM-receptor interaction. We discuss the relationship between the observed gene expression profiles and PrPSc deposition and the potential involvement in the pathogenesis of scrapie of 7 specific differentially expressed genes whose expression levels were confirmed by real time-PCR.
Conclusions:
The present findings identify new genes that may be involved in the pathogenesis of natural scrapie infection in the lymphoreticular system, and confirm previous reports describing scrapie-induced alterations in the expression of genes involved in protein misfolding, angiogenesis and the oxidative stress response. Further studies will be necessary to determine the role of these genes in prion replication, dissemination and in the response of the organism to this disease.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-59
Año: 2014
Publicado en: BMC Genomics 15, 59 (2014), 1-17
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2008-0256
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Sanidad Animal (Departamento de Patología Animal)
Área (Departamento): Genética (Departamento de Anatomía, Embriología y Genética Animal)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. Exportado de SIDERAL (2017-12-11-08:30:08)
Este artículo se encuentra en las siguientes colecciones:
Artículos > Artículos por área > Sanidad Animal
Artículos > Artículos por área > Genética
Registro creado el 2017-07-13, última modificación el 2017-12-11