@article{GuevaraRodríguez:63647,
author = "Guevara Rodríguez, Susana and Schoorlemmer, Jon and
Climent Aroz, María",
title = "{Regulación Epigenética del locus Cd9 por REX1}",
year = "2017",
note = "The transcription factor REX1 controls the methylation
levels of imprinted genes during early embryogenesis and
participates in the silencing of endogenous retroviruses
(ERVs). It has been found by ChiPseq that this factor
targets the Cd9 gene which encodes a protein with the same
name that participates in multiple biological activities.
This relationship suggests that REX1 act as a
transcriptional regulator of Cd9 by epigenetic mechanisms.
In the present work, we studied whether REX1 is able to
modulate the expression of CD9 locus. To this end, the
percent methylation of Cd9 genomic sequences close to the
ChiPseq peak and the levels of RNA expression of the Cd9
locus by qPCR were determined. We analyzed cells and tissue
samples from mouse (ES, TS, placentas and embryos) wt and
compared them with REX1 - / - and REX1 +/-. The results
indicated that REX1 acts as a protector of methylation of
the Cd9 locus and repressor of its expression. This effect
seems to be unrelated because there is no negative
correlation between the degree of methylation of the
analysed locus in these tissues and the levels of Cd9
expression. It is possible that this region is not
determinant for the control of its expression, or other
regulatory mechanisms of transcription are acting.",
}