Effect of pressure-induced changes in the ionization equilibria of buffers on inactivation of Escherichia coli and staphylococcus aureus by high hydrostatic pressure

Gayán, E. (Universidad de Zaragoza) ; Condón, S. (Universidad de Zaragoza) ; Álvarez, I. (Universidad de Zaragoza) ; Nabakabaya, M. ; Mackey, B.

Resumen: Survival rates of Escherichia coli and Staphylococcus aureus after high-pressure treatment in buffers that had large or small reaction volumes (¿V°), and which therefore underwent large or small changes in pH under pressure, were compared. At a low buffer concentration of 0.005 M, survival was, as expected, better in MOPS (morpholinepropanesulfonic acid), HEPES, and Tris, whose ¿V° values are approximately 5.0 to 7.0 cm3 mol-1, than in phosphate or dimethyl glutarate (DMG), whose ¿V° values are about -25 cm3 mol-1. However, at a concentration of 0.1 M, survival was unexpectedly better in phosphate and DMG than in MOPS, HEPES, or Tris. This was because the baroprotective effect of phosphate and DMG increased much more rapidly with increasing concentration than it did with MOPS, HEPES, or Tris. Further comparisons of survival in solutions of salts expected to cause large electrostriction effects (Na2SO4 and CaCl2) and those causing lower electrostriction (NaCl and KCl) were made. The salts with divalent ions were protective at much lower concentrations than salts with monovalent ions. Buffers and salts both protected against transient membrane disruption in E. coli, but the molar concentrations necessary for membrane protection were much lower for phosphate and Na2SO4 than for HEPES and NaCl. Possible protective mechanisms discussed include effects of electrolytes on water compressibility and kosmotropic and specific ion effects. The results of this systematic study will be of considerable practical significance in studies of pressure inactivation of microbes under defined conditions but also raise important fundamental questions regarding the mechanisms of baroprotection by ionic solutes.

Idioma: Inglés

DOI: 10.1128/AEM.00469-13

Año: 2013

Publicado en: APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79, 13 (2013), 4041-4047
ISSN: 0099-2240

Factor impacto JCR: 3.952 (2013)

Categ. JCR: MICROBIOLOGY rank: 24 / 119 = 0.202 (2013) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 30 / 165 = 0.182 (2013) - Q1 - T1

Financiación: info:eu-repo/grantAgreement/ES/CICYT-FEDER/CIT-020000-2009-40

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)