Nanoparticles engineered to bind cellular motors for efficient delivery

Dalmau-Mena, I. ; del Pino, P. ; Pelaz, B. ; Cuesta-Geijo, M. ; Galindo, I. ; Moros, M. (Universidad de Zaragoza) ; de la Fuente, J.M. (Universidad de Zaragoza) ; Alonso, C.

Resumen: Background: Dynein is a cytoskeletal molecular motor protein that transports cellular cargoes along microtubules. Biomimetic synthetic peptides designed to bind dynein have been shown to acquire dynamic properties such as cell accumulation and active intra- and inter-cellular motion through cell-to-cell contacts and projections to distant cells. On the basis of these properties dynein-binding peptides could be used to functionalize nanoparticles for drug delivery applications. Results: Here, we show that gold nanoparticles modified with dynein-binding delivery sequences become mobile, powered by molecular motor proteins. Modified nanoparticles showed dynamic properties, such as travelling the cytosol, crossing intracellular barriers and shuttling the nuclear membrane. Furthermore, nanoparticles were transported from one cell to another through cell-to-cell contacts and quickly spread to distant cells through cell projections. Conclusions: The capacity of these motor-bound nanoparticles to spread to many cells and increasing cellular retention, thus avoiding losses and allowing lower dosage, could make them candidate carriers for drug delivery.

Idioma: Inglés

DOI: 10.1186/s12951-018-0354-1

Año: 2018

Publicado en: Journal of Nanobiotechnology 16, 1 (2018), 33 [13 pp]
ISSN: 1477-3155

Factor impacto JCR: 5.345 (2018)

Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 19 / 162 = 0.117 (2018) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 30 / 94 = 0.319 (2018) - Q2 - T1

Factor impacto SCIMAGO: 1.255 - Applied Microbiology and Biotechnology (Q1) - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmaceutical Science (Q1) - Molecular Medicine (Q1) - Nanoscience and Nanotechnology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/COST/CA15138 Transautophagy
Financiación: info:eu-repo/grantAgreement/EC/FP7/239931/EU/Multifunctional Magnetic Nanoparticles: Towards Smart Drugs Design/NANOPUZZLE
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2012-34533
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69598-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)