The intrinsic fluorescence of FAD and its application in analytical chemistry: a review

Galban, J. (Universidad de Zaragoza) ; Sanz-Vicente, I. (Universidad de Zaragoza) ; Navarro, J. (Universidad de Zaragoza) ; de Marcos, S. (Universidad de Zaragoza)

Resumen: This review (with 106 references) mainly deals with the analytical applications of flavin-adenine dinucleotide (FAD) fluorescence. In the first section, the spectroscopic properties of this compound are reviewed at the light of his different acid-base, oxidation and structural forms; the chemical and spectroscopic properties of flavin mononucleotide (FMN) and other flavins will be also briefly discussed. The second section discusses how the properties of FAD fluorescence changes in flavoenzymes (FvEs), again considering the different chemical and structural forms; the glucose oxidase (GOx) and the choline oxidase (ChOx) cases will be commented. Since almost certainly the most reported analytical application of FAD fluorescence is as an auto-indicator in enzymatic methods catalysed by FvE oxidoreductases, it is important to know how the concentrations of the different forms of FAD changes along the reaction and, consequently, the fluorescence and the analytical signals. An approach to do this will be presented in section 3. The fourth part of the paper compiles the analytical applications which have been reported until now based in these fluorescence properties. Finally, some suggestions about tentative future research are also given.

Idioma: Inglés

DOI: 10.1088/2050-6120/4/4/042005

Año: 2016

Publicado en: Methods and Applications in Fluorescence 4, 4 (2016), 042005 [19 pp]
ISSN: 2050-6120

Factor impacto JCR: 2.656 (2016)

Categ. JCR: CHEMISTRY, PHYSICAL rank: 66 / 145 = 0.455 (2016) - Q2 - T2
Categ. JCR: CHEMISTRY, ANALYTICAL rank: 27 / 76 = 0.355 (2016) - Q2 - T2

Factor impacto SCIMAGO: 0.895 - Atomic and Molecular Physics, and Optics (Q1) - Instrumentation (Q1) - Materials Science (miscellaneous) (Q1) - Spectroscopy (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E74
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774

Tipo y forma: Review (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)