Comparative effect of growth media on the monitoring of E. coli inactivation and regrowth after solar and photo-Fenton treatment

Valero, P. (Universidad de Zaragoza) ; Giannakis, S. ; Mosteo, R. (Universidad de Zaragoza) ; Ormad, M. P. (Universidad de Zaragoza) ; Pulgarin, C.

Resumen: This study intends to assess the cultivability of photo-treated Escherichia coli K-12 on media with different selectivity (PCA, LBA, T-7, T-7 + TTC, VRBA and MAC) and to establish optimal conditions for bacterial recuperation. For these purposes, immediate and long-term bacterial recovery after SODIS and photo-Fenton are evaluated. Moreover, the use of catalase and sodium pyruvate supplements in the medium is studied. The non-selective medium PCA showed the highest counts for the untreated and treated cells due to its content in nutrients (e.g. glucose) and lack of inhibitors. On the contrary, the selective media showed lower recovery, being the culture media effectiveness: PCA > LBA > T-7 > T-7 + TTC > VRBA > MAC. The presence of inhibitors, such as heptadecyl sulfate in T-7 or bile salts in VRBA and MAC, reduced the cultivability of the treated cells. These compounds can probably diffuse into the cells more easily after SODIS and photo-Fenton as a consequence of the loss of the membrane integrity. In addition, the lack of yeast extract in MAC had a detrimental effect on E. coli recovery. Sodium pyruvate was tested as supplement to PCA, leading to slightly enhanced bacterial immediate recovery after SODIS, SODIS + H2O2 and photo-Fenton. The addition of catalase and sodium pyruvate to the bulk was studied as well, considerably increasing bacterial survival in the long-term due to their ability to neutralize residual H2O2.

Idioma: Inglés

DOI: 10.1016/j.cej.2016.11.126

Año: 2017

Publicado en: Chemical Engineering Journal 313 (2017), 109-120
ISSN: 1385-8947

Factor impacto JCR: 6.735 (2017)

Categ. JCR: ENGINEERING, ENVIRONMENTAL rank: 3 / 50 = 0.06 (2017) - Q1 - T1
Categ. JCR: ENGINEERING, CHEMICAL rank: 7 / 137 = 0.051 (2017) - Q1 - T1

Factor impacto SCIMAGO: 1.863 - Chemical Engineering (miscellaneous) (Q1) - Industrial and Manufacturing Engineering (Q1) - Environmental Chemistry (Q1) - Chemistry (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/EC/H2020/688928/EU/Water - Sustainable Point-Of-Use Treatment Technologies/WATERSPOUTT

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)