An Alternative Homodimerization Interface of MnmG Reveals a Conformational Dynamics that Is Essential for Its tRNA Modification Function

Ruiz-Partida, R. ; Prado, S. ; Villarroya, M. ; Velázquez-Campoy, A. (Universidad de Zaragoza) ; Bravo, J. ; Armengod, M.E.

Resumen: The Escherichia coli homodimeric proteins MnmE and MnmG form a functional complex, MnmEG, that modifies tRNAs using GTP, methylene-tetrahydrofolate, FAD, and glycine or ammonium. MnmE is a tetrahydrofolate- and GTP-binding protein, whereas MnmG is a FAD-binding protein with each protomer composed of the FAD-binding domain, two insertion domains, and the helical C-terminal domain. The detailed mechanism of the MnmEG-mediated reaction remains unclear partially due to incomplete structural information on the free- and substrate-bound forms of the complex. In this study, we show that MnmG can adopt in solution a dimer arrangement (form I) different from that currently considered as the only biologically active (form II). Normal mode analysis indicates that form I can oscillate in a range of open and closed conformations. Using isothermal titration calorimetry and native red electrophoresis, we show that a form-I open conformation, which can be stabilized in vitro by the formation of an interprotomer disulfide bond between the catalytic C277 residues, appears to be involved in the assembly of the MnmEG catalytic center. We also show that residues R196, D253, R436, R554 and E585 are important for the stabilization of form I and the tRNA modification function. We propose that the form I dynamics regulates the alternative access of MnmE and tRNA to the MnmG FAD active site. Finally, we show that the C-terminal region of MnmG contains a sterile alpha motif domain responsible for tRNA–protein and protein–protein interactions.

Idioma: Inglés

DOI: 10.1016/j.jmb.2018.05.035

Año: 2018

Publicado en: JOURNAL OF MOLECULAR BIOLOGY 430 (2018), 2822-2842
ISSN: 0022-2836

Factor impacto JCR: 5.067 (2018)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 47 / 294 = 0.16 (2018) - Q1 - T1

Factor impacto SCIMAGO: 3.578 - Structural Biology (Q1) - Molecular Biology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2010-19737
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2014-58673-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P

Tipo y forma: Article (Published version)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)