000076038 001__ 76038 000076038 005__ 20190709135455.0 000076038 0247_ $$2doi$$a10.1016/j.anireprosci.2017.03.022 000076038 0248_ $$2sideral$$a98976 000076038 037__ $$aART-2017-98976 000076038 041__ $$aeng 000076038 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.L.$$uUniversidad de Zaragoza 000076038 245__ $$aToward an integrative and predictive sperm quality analysis in Bos taurus 000076038 260__ $$c2017 000076038 5060_ $$aAccess copy available to the general public$$fUnrestricted 000076038 5203_ $$aThere is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS®3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on membrane and acrosomal integrity, motility and morphology. It was also possible to observe live spermatozoa showing signs of capacitation such as hyperactivated motility and changes in acrosomal structure. Sperm subpopulation with intact plasma membrane and acrosome showed a higher proportion of motile sperm than those with damaged acrosome or increased fluorescence intensity. Spermatozoa with intact plasmalemma and damaged acrosome were static or exhibit weak movement. Significant correlations among the different sperm quality parameters evaluated were also described. We concluded that the ISAS®3Fun is an integrated method that represents an advance in sperm quality analysis with the potential to improve fertility predictions. 000076038 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P 000076038 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000076038 590__ $$a1.647$$b2017 000076038 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b14 / 59 = 0.237$$c2017$$dQ1$$eT1 000076038 591__ $$aREPRODUCTIVE BIOLOGY$$b22 / 29 = 0.759$$c2017$$dQ4$$eT3 000076038 592__ $$a0.704$$b2017 000076038 593__ $$aAnimal Science and Zoology$$c2017$$dQ1 000076038 593__ $$aFood Animals$$c2017$$dQ1 000076038 593__ $$aMedicine (miscellaneous)$$c2017$$dQ2 000076038 593__ $$aEndocrinology$$c2017$$dQ3 000076038 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000076038 700__ $$aSoler, C. 000076038 700__ $$0(orcid)0000-0003-0389-2422$$aAlquézar-Baeta, C.$$uUniversidad de Zaragoza 000076038 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza 000076038 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología 000076038 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000076038 773__ $$g181 (2017), 108-114$$pAnim. reprod. sci.$$tANIMAL REPRODUCTION SCIENCE$$x0378-4320 000076038 8564_ $$s501121$$uhttps://zaguan.unizar.es/record/76038/files/texto_completo.pdf$$yPostprint 000076038 8564_ $$s45066$$uhttps://zaguan.unizar.es/record/76038/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000076038 909CO $$ooai:zaguan.unizar.es:76038$$particulos$$pdriver 000076038 951__ $$a2019-07-09-11:43:04 000076038 980__ $$aARTICLE