000076038 001__ 76038
000076038 005__ 20190709135455.0
000076038 0247_ $$2doi$$a10.1016/j.anireprosci.2017.03.022
000076038 0248_ $$2sideral$$a98976
000076038 037__ $$aART-2017-98976
000076038 041__ $$aeng
000076038 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.L.$$uUniversidad de Zaragoza
000076038 245__ $$aToward an integrative and predictive sperm quality analysis in Bos taurus
000076038 260__ $$c2017
000076038 5060_ $$aAccess copy available to the general public$$fUnrestricted
000076038 5203_ $$aThere is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS®3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on membrane and acrosomal integrity, motility and morphology. It was also possible to observe live spermatozoa showing signs of capacitation such as hyperactivated motility and changes in acrosomal structure. Sperm subpopulation with intact plasma membrane and acrosome showed a higher proportion of motile sperm than those with damaged acrosome or increased fluorescence intensity. Spermatozoa with intact plasmalemma and damaged acrosome were static or exhibit weak movement. Significant correlations among the different sperm quality parameters evaluated were also described. We concluded that the ISAS®3Fun is an integrated method that represents an advance in sperm quality analysis with the potential to improve fertility predictions.
000076038 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
000076038 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000076038 590__ $$a1.647$$b2017
000076038 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b14 / 59 = 0.237$$c2017$$dQ1$$eT1
000076038 591__ $$aREPRODUCTIVE BIOLOGY$$b22 / 29 = 0.759$$c2017$$dQ4$$eT3
000076038 592__ $$a0.704$$b2017
000076038 593__ $$aAnimal Science and Zoology$$c2017$$dQ1
000076038 593__ $$aFood Animals$$c2017$$dQ1
000076038 593__ $$aMedicine (miscellaneous)$$c2017$$dQ2
000076038 593__ $$aEndocrinology$$c2017$$dQ3
000076038 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000076038 700__ $$aSoler, C.
000076038 700__ $$0(orcid)0000-0003-0389-2422$$aAlquézar-Baeta, C.$$uUniversidad de Zaragoza
000076038 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000076038 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000076038 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000076038 773__ $$g181 (2017), 108-114$$pAnim. reprod. sci.$$tANIMAL REPRODUCTION SCIENCE$$x0378-4320
000076038 8564_ $$s501121$$uhttps://zaguan.unizar.es/record/76038/files/texto_completo.pdf$$yPostprint
000076038 8564_ $$s45066$$uhttps://zaguan.unizar.es/record/76038/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000076038 909CO $$ooai:zaguan.unizar.es:76038$$particulos$$pdriver
000076038 951__ $$a2019-07-09-11:43:04
000076038 980__ $$aARTICLE