Effects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°c

Domingo, P. ; Olaciregui, M. ; González, N. (Universidad de Zaragoza) ; De Blas, I. (Universidad de Zaragoza) ; Gil, L. (Universidad de Zaragoza)

Resumen: The purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16°C for 72 h and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP. Each sample was then resuspended with extender A, B, C, or D, respectively. All samples were stored at 16°C and analysed at 4, 24, 48, and 72 h by ISAS®, vitality test, HOS test, and acrosome integrity test. After analyse of the results, SP samples showed a significantly higher percentage (P=0.020) in the HOS test (71.9 ± 1.6%) than non-SP samples (66.5 ± 1.6%). Non-SP samples had better results for kinematic parameters. Extenders A and C showed great results for the percentage of motile spermatozoa (63.1 ± 4.3% and 63.4 ± 3.7%, respectively), vitality (88.9 ± 2.6% and 87.7 ± 2.7%, respectively), and HOS test (68.9 ± 1.4% and 75.2 ± 1.4%, respectively). Extenders B and D showed worse data for sperm quality. These results suggest that SP has a protective effect on rabbit sperm membranes and maintains better sperm motility. The addition of glycerol and NMP to INRA does not improve rabbit sperm quality; nevertheless, the DMF cryoprotectant exerts a protective effect on the membrane of spermatozoa, improving seminal quality during rabbit sperm preservation at 16°C.

Idioma: Inglés

DOI: 10.1538/expanim.17-0152

Año: 2018

Publicado en: EXPERIMENTAL ANIMALS 67, 4 (2018), 413-420
ISSN: 1341-1357

Factor impacto JCR: 1.208 (2018)

Categ. JCR: ZOOLOGY rank: 76 / 170 = 0.447 (2018) - Q2 - T2
Categ. JCR: VETERINARY SCIENCES rank: 54 / 141 = 0.383 (2018) - Q2 - T2

Factor impacto SCIMAGO: 0.5 - Animal Science and Zoology (Q2) - Veterinary (miscellaneous) (Q2) - Medicine (miscellaneous) (Q2) - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/FSE

Tipo y forma: Article (Published version)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)