Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot

Herrera, S. ; Rodrigo, J. ; Hormaza, J.I. ; Lora, J.

Resumen: Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-Incompatibility (GSI) system. Incompatibility relationships between cultivars can be established by an S-allele genotyping PCR strategy. Until recently, most of the traditional European apricot cultivars were self-compatible but several breeding programs have introduced an increasing number of new cultivars whose pollination requirements are unknown. To fill this gap, we have identified the S-allele of 44 apricot genotypes, of which 43 are reported here for the first time. The identification of S-c in 15 genotypes suggests that those cultivars are self-compatible. In five genotypes, self-(in)compatibility was established by the observation of pollen tube growth in self-pollinated flowers, since PCR analysis could not allowed distinguishing between the S-c and S-8 alleles. Self-incompatible genotypes were assigned to their corresponding self-incompatibility groups. The knowledge of incompatibility relationships between apricot cultivars can be a highly valuable tool for the development of future breeding programs by selecting the appropriate parents and for efficient orchard design by planting self-compatible and inter-compatible cultivars.

Idioma: Inglés

DOI: 10.3390/ijms19113612

Año: 2018

Publicado en: International Journal of Molecular Sciences 19, 11 (2018), 3612 [13 pp]
ISSN: 1661-6596

Factor impacto JCR: 4.183 (2018)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 45 / 172 = 0.262 (2018) - Q2 - T1
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 77 / 294 = 0.262 (2018) - Q2 - T1

Factor impacto SCIMAGO: 1.312 - Catalysis (Q1) - Computer Science Applications (Q1) - Inorganic Chemistry (Q1) - Spectroscopy (Q1) - Molecular Biology (Q1) - Organic Chemistry (Q1) - Physical and Theoretical Chemistry (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A12-17R
Financiación: info:eu-repo/grantAgreement/ES/INIA/RFP2015-00015-00
Financiación: info:eu-repo/grantAgreement/ES/INIA/RTA2014-00085-00
Financiación: info:eu-repo/grantAgreement/ES/INIA/RTA2017-00003-00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/AGL2015-74071-JIN
Financiación: info:eu-repo/grantAgreement/ES/MICINN/AGL2016-77267-R

Tipo y forma: Artículo (Versión definitiva)