Gold nanoclusters as a quenchable fluorescent probe for sensing oxygen at high temperatures

Martín-Barreiro, A. ; Marcos, S. de (Universidad de Zaragoza) ; Galbán, J. (Universidad de Zaragoza)

Resumen: Gold nanoclusters (AuNCs) capped with lipoic acid (LA) or templated with bovine serum albumin (BSA) are shown to be viable fluorescent probes for oxygen (O2) which acts as a collisional quencher. Quenching of fluorescence, with its lifetimes in the order of 123 ± 9 ns (LA) and 153 ± 15 ns (BSA) (in aqueous solution), is best measured at excitation/emission wavelengths of 400/680 nm and 375/650 nm respectively. It follows the Stern-Volmer model, whose quenching constants (Ksv) and quenching efficiencies (¿) are 1400 M-1 and 0.52 for AuNC@LA and 4479 M-1 and 0.90 for AuNC@BSA. The probes were immobilized on a silica support and tested for response to O2 in gas phase using a commercial instrument. The effect of temperature on the fluorescence of AuNC@LA was studied in the range from 30 to 210 °C. Fluorescence intensity slightly decreases with temperature in the first heating cycle but remains constant in further cycles. The AuNC@LA were studied for their response to O2 in the temperature range from 30 to 100 °C, and even at 100 °C they respond to O2, with a Ksv that slightly drops with increasing temperature. Measuring in gas phase at 100 °C, the sensor has a detection limit of 3% (V/V) of O2 at a signal-to-noise ratio of 3. [Figure not available: see fulltext.].

Idioma: Inglés

DOI: 10.1007/s00604-018-2676-y

Año: 2018

Publicado en: Mikrochimica acta 185, 3 (2018), 171 [7 pp]
ISSN: 0026-3672

Factor impacto JCR: 5.479 (2018)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 9 / 84 = 0.107 (2018) - Q1 - T1

Factor impacto SCIMAGO: 1.243 - Analytical Chemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E74
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)