Exosome origin determines cell targeting and the transfer of therapeutic nanoparticles towards target cells
Sancho-Albero, M. (Universidad de Zaragoza) ; Navascués, N. ; Mendoza, G. (Universidad de Zaragoza) ; Sebastián, V. (Universidad de Zaragoza) ; Arruebo, M. (Universidad de Zaragoza) ; Martín-Duque, P. ; Santamaría, J. (Universidad de Zaragoza)
Resumen: Background: Exosomes are considered key elements for communication between cells, but very little is known about the mechanisms and selectivity of the transference processes involving exosomes released from different cells.
Results: In this study we have investigated the transfer of hollow gold nanoparticles (HGNs) between different cells when these HGNs were loaded within exosomes secreted by human placental mesenchymal stem cells (MSCs). These HGNs were successfully incorporated in the MSCs exosome biogenesis pathway and released as HGNs-loaded exosomes. Time-lapse microscopy and atomic emission spectroscopy allowed us to demonstrate the selective transfer of the secreted exosomes only to the cell type of origin when studying different cell types including cancer, metastatic, stem or immunological cells.
Conclusions: In this study we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Specifically, we show how this preferential uptake can be leveraged to selectively induce cell death by light-induced hyperthermia only in cells of the same type as those producing the corresponding loaded exosomes. We describe how the exosomes are preferentially transferred to some cell types but not to others, thus providing a better understanding to design selective therapies for different diseases.[Figure not available: see fulltext.]
Idioma: Inglés
DOI: 10.1186/s12951-018-0437-z
Año: 2019
Publicado en: Journal of Nanobiotechnology 17, 1 (2019), 16 [13 pp]
ISSN: 1477-3155
Factor impacto JCR: 6.518 (2019)
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 15 / 156 = 0.096 (2019) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 30 / 103 = 0.291 (2019) - Q2 - T1
Factor impacto SCIMAGO: 1.335 - Applied Microbiology and Biotechnology (Q1) - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmaceutical Science (Q1) - Medicine (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q2) - Molecular Medicine (Q2)
Financiación: info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
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Results: In this study we have investigated the transfer of hollow gold nanoparticles (HGNs) between different cells when these HGNs were loaded within exosomes secreted by human placental mesenchymal stem cells (MSCs). These HGNs were successfully incorporated in the MSCs exosome biogenesis pathway and released as HGNs-loaded exosomes. Time-lapse microscopy and atomic emission spectroscopy allowed us to demonstrate the selective transfer of the secreted exosomes only to the cell type of origin when studying different cell types including cancer, metastatic, stem or immunological cells.
Conclusions: In this study we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Specifically, we show how this preferential uptake can be leveraged to selectively induce cell death by light-induced hyperthermia only in cells of the same type as those producing the corresponding loaded exosomes. We describe how the exosomes are preferentially transferred to some cell types but not to others, thus providing a better understanding to design selective therapies for different diseases.[Figure not available: see fulltext.]
Idioma: Inglés
DOI: 10.1186/s12951-018-0437-z
Año: 2019
Publicado en: Journal of Nanobiotechnology 17, 1 (2019), 16 [13 pp]
ISSN: 1477-3155
Factor impacto JCR: 6.518 (2019)
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 15 / 156 = 0.096 (2019) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 30 / 103 = 0.291 (2019) - Q2 - T1
Factor impacto SCIMAGO: 1.335 - Applied Microbiology and Biotechnology (Q1) - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmaceutical Science (Q1) - Medicine (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q2) - Molecular Medicine (Q2)
Financiación: info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. Exportado de SIDERAL (2021-01-08-08:04:02)
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Registro creado el 2019-02-19, última modificación el 2021-01-08