000077170 001__ 77170
000077170 005__ 20210108084705.0
000077170 0247_ $$2doi$$a10.1186/s12951-018-0437-z
000077170 0248_ $$2sideral$$a110377
000077170 037__ $$aART-2019-110377
000077170 041__ $$aeng
000077170 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, M.$$uUniversidad de Zaragoza
000077170 245__ $$aExosome origin determines cell targeting and the transfer of therapeutic nanoparticles towards target cells
000077170 260__ $$c2019
000077170 5060_ $$aAccess copy available to the general public$$fUnrestricted
000077170 5203_ $$aBackground: Exosomes are considered key elements for communication between cells, but very little is known about the mechanisms and selectivity of the transference processes involving exosomes released from different cells.
Results: In this study we have investigated the transfer of hollow gold nanoparticles (HGNs) between different cells when these HGNs were loaded within exosomes secreted by human placental mesenchymal stem cells (MSCs). These HGNs were successfully incorporated in the MSCs exosome biogenesis pathway and released as HGNs-loaded exosomes. Time-lapse microscopy and atomic emission spectroscopy allowed us to demonstrate the selective transfer of the secreted exosomes only to the cell type of origin when studying different cell types including cancer, metastatic, stem or immunological cells.
Conclusions: In this study we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Specifically, we show how this preferential uptake can be leveraged to selectively induce cell death by light-induced hyperthermia only in cells of the same type as those producing the corresponding loaded exosomes. We describe how the exosomes are preferentially transferred to some cell types but not to others, thus providing a better understanding to design selective therapies for different diseases.[Figure not available: see fulltext.]
000077170 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN$$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
000077170 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000077170 590__ $$a6.518$$b2019
000077170 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b15 / 156 = 0.096$$c2019$$dQ1$$eT1
000077170 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b30 / 103 = 0.291$$c2019$$dQ2$$eT1
000077170 592__ $$a1.335$$b2019
000077170 593__ $$aApplied Microbiology and Biotechnology$$c2019$$dQ1
000077170 593__ $$aBioengineering$$c2019$$dQ1
000077170 593__ $$aBiomedical Engineering$$c2019$$dQ1
000077170 593__ $$aPharmaceutical Science$$c2019$$dQ1
000077170 593__ $$aMedicine (miscellaneous)$$c2019$$dQ1
000077170 593__ $$aNanoscience and Nanotechnology$$c2019$$dQ2
000077170 593__ $$aMolecular Medicine$$c2019$$dQ2
000077170 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000077170 700__ $$0(orcid)0000-0003-3363-2912$$aNavascués, N.
000077170 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, G.$$uUniversidad de Zaragoza
000077170 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, V.$$uUniversidad de Zaragoza
000077170 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000077170 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, P.
000077170 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, J.$$uUniversidad de Zaragoza
000077170 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000077170 773__ $$g17, 1 (2019), 16 [13 pp]$$pJ. nanobiotechnol.$$tJournal of Nanobiotechnology$$x1477-3155
000077170 8564_ $$s1725510$$uhttps://zaguan.unizar.es/record/77170/files/texto_completo.pdf$$yVersión publicada
000077170 8564_ $$s63975$$uhttps://zaguan.unizar.es/record/77170/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000077170 909CO $$ooai:zaguan.unizar.es:77170$$particulos$$pdriver
000077170 951__ $$a2021-01-08-08:04:02
000077170 980__ $$aARTICLE