000077187 001__ 77187
000077187 005__ 20200716101516.0
000077187 0247_ $$2doi$$a10.1039/C8RA09088B
000077187 0248_ $$2sideral$$a110411
000077187 037__ $$aART-2019-110411
000077187 041__ $$aeng
000077187 100__ $$0(orcid)0000-0003-1276-5927$$aHernandez, Y.
000077187 245__ $$aGold nanoparticle coatings as efficient adenovirus carriers to non-infectable stem cells
000077187 260__ $$c2019
000077187 5060_ $$aAccess copy available to the general public$$fUnrestricted
000077187 5203_ $$aMesenchymal stem cells (MSCs) are adult pluripotent cells with the plasticity to be converted into different cell types. Their self-renewal capacity, relative ease of isolation, expansion and inherent migration to tumors, make them perfect candidates for cell therapy against cancer. However, MSCs are notoriously refractory to adenoviral infection, mainly because CAR (Coxsackie-Adenovirus Receptor) expression is absent or downregulated. Over the last years, nanoparticles have attracted a great deal of attention as potential vehicle candidates for gene delivery, but with limited effects on their own. Our data showed that the use of positively charged 14 nm gold nanoparticles either functionalized with arginine-glycine-aspartate (RGD) motif or not, increases the efficiency of adenovirus infection in comparison to commercial reagents without altering cell viability or cell phenotype. This system represents a simple, efficient and safe method for the transduction of MSCs, being attractive for cancer gene and cell therapies.
000077187 536__ $$9info:eu-repo/grantAgreement/EC/FP7/219433/EU/Transnational call for collaborative proposals in basic nanoscience research/NANOSCI-EPLUS$$9info:eu-repo/grantAgreement/ES/DGA/B054-12
000077187 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000077187 590__ $$a3.119$$b2019
000077187 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b73 / 177 = 0.412$$c2019$$dQ2$$eT2
000077187 592__ $$a0.736$$b2019
000077187 593__ $$aChemistry (miscellaneous)$$c2019$$dQ1
000077187 593__ $$aChemical Engineering (miscellaneous)$$c2019$$dQ1
000077187 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000077187 700__ $$0(orcid)0000-0002-5630-6645$$aGonzález-Pastor, R.
000077187 700__ $$aBelmar-Lopez, C.
000077187 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, G.$$uUniversidad de Zaragoza
000077187 700__ $$0(orcid)0000-0003-1081-8482$$aDe La Fuente, J.M.
000077187 700__ $$0(orcid)0000-0003-4160-9720$$aMartin-Duque, P.
000077187 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000077187 773__ $$g9, 3 (2019), 1327-1334$$pRSC ADVANCES$$tRSC Advances$$x2046-2069
000077187 8564_ $$s440423$$uhttps://zaguan.unizar.es/record/77187/files/texto_completo.pdf$$yVersión publicada
000077187 8564_ $$s118244$$uhttps://zaguan.unizar.es/record/77187/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000077187 909CO $$ooai:zaguan.unizar.es:77187$$particulos$$pdriver
000077187 951__ $$a2020-07-16-09:22:58
000077187 980__ $$aARTICLE