SPIONs' Enhancer Effect on Cell Transfection: An Unexpected Advantage for an Improved Gene Delivery System
Balcells, L. ; Fornaguera, C. ; Brugada-Vilà, P. ; Guerra-Rebollo, M. ; Meca-Cortés, Ó. ; Martínez, G. (Universidad de Zaragoza) ; Rubio, N. ; Blanco, J. ; Santamaría, J. (Universidad de Zaragoza) ; Cascante, A. ; Borrós, S.
Resumen: The clinical implantation of gene therapy is hindered by the limitations of current gene delivery vectors, namely, safety issues regarding viral vectors and low transfection efficacy regarding nonviral vectors. Thus, the design of safe and efficient gene carriers is a key point for the success of such therapies. In addition, when employing genetically modified cells for further applications, the selection of successfully modified cells becomes crucial. To address these issues, we have developed multicomponent nanoparticles composed of poly(ß-amino ester) (pBAE) polymers, plasmid DNA, and superparamagnetic iron oxide nanoparticles (SPIONs). Whereas pBAEs were initially employed as safe and biocompatible carriers with improved transfection efficiency, as compared to commercial vectors, SPIONs were used because of their magnetic character that enables cell selection. Surprisingly, the results presented here revealed an unexpected enhancer effect of SPIONs on the transfection efficiency of pBAE/pDNA polyplexes in both permissive and reluctant to transfection cell lines. This unanticipated outcome, together with the allowance to perform a selective magnetic sorting of genetically modified cells without interfering in cell transfection, opens the door to SPION-containing nanoparticles as promising tools for cell therapy approaches.
Idioma: Inglés
DOI: 10.1021/acsomega.8b02905
Año: 2019
Publicado en: ACS OMEGA 4, 2 (2019), 2728-2740
ISSN: 2470-1343
Factor impacto JCR: 2.87 (2019)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 81 / 176 = 0.46 (2019) - Q2 - T2
Factor impacto SCIMAGO: 0.767 - Chemistry (miscellaneous) (Q1) - Chemical Engineering (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/RTC-2015-3751-1
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-2-R
Tipo y forma: Article (Published version)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Idioma: Inglés
DOI: 10.1021/acsomega.8b02905
Año: 2019
Publicado en: ACS OMEGA 4, 2 (2019), 2728-2740
ISSN: 2470-1343
Factor impacto JCR: 2.87 (2019)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 81 / 176 = 0.46 (2019) - Q2 - T2
Factor impacto SCIMAGO: 0.767 - Chemistry (miscellaneous) (Q1) - Chemical Engineering (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/RTC-2015-3751-1
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER-ISCIII/SAF2015-64927-C2-2-R
Tipo y forma: Article (Published version)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2023-09-21-13:29:01)
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Record created 2019-03-13, last modified 2023-09-21