Tunable injectable alginate-based hydrogel for cell therapy in Type 1 Diabetes Mellitus

Espona-Noguera, A. ; Ciriza, J. ; Cañibano-Hernández, A. ; Fernandez, L. ; Ochoa, I. (Universidad de Zaragoza) ; Saenz del Burgo, L. ; Pedraz, J.L.

Resumen: Islet transplantation has the potential of reestablishing naturally-regulated insulin production in Type 1 diabetic patients. Nevertheless, this procedure is limited due to the low islet survival after transplantation and the lifelong immunosuppression to avoid rejection. Islet embedding within a biocompatible matrix provides mechanical protection and a physical barrier against the immune system thus, increasing islet survival. Alginate is the preferred biomaterial used for embedding insulin-producing cells because of its biocompatibility, low toxicity and ease of gelation. However, alginate gelation is poorly controlled, affecting its physicochemical properties as an injectable biomaterial. Including different concentrations of the phosphate salt Na2HPO4 in alginate hydrogels, we can modulate their gelation time, tuning their physicochemical properties like stiffness and porosity while maintaining an appropriate injectability. Moreover, these hydrogels showed good biocompatibility when embedding a rat insulinoma cell line, especially at low Na2HPO4 concentrations, indicating that these hydrogels have potential as injectable biomaterials for Type 1 Diabetes Mellitus treatment.

Idioma: Inglés

DOI: 10.1016/j.ijbiomac.2017.09.103

Año: 2018

Publicado en: International journal of biological macromolecules 107, PartA (2018), 1261-1269
ISSN: 0141-8130

Factor impacto JCR: 4.784 (2018)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 52 / 294 = 0.177 (2018) - Q1 - T1
Categ. JCR: POLYMER SCIENCE rank: 7 / 85 = 0.082 (2018) - Q1 - T1
Categ. JCR: CHEMISTRY, APPLIED rank: 9 / 70 = 0.129 (2018) - Q1 - T1

Factor impacto SCIMAGO: 0.962 - Biochemistry (Q1) - Structural Biology (Q1) - Molecular Biology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/T88
Financiación: info:eu-repo/grantAgreement/EC/H2020/645991/EU/Diabetes Reversing Implants with enhanced Viability and long-term Efficacy/DRIVE
Financiación: info:eu-repo/grantAgreement/ES/ISCII-CIBER/DRUGDIFF
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)