Intracellular delivery of biologically-active fungal metabolite gliotoxin using magnetic nanoparticles

Comas, Laura ; Polo, Esther ; Domingo, M. Pilar ; Hernandez, Yulán ; Arias, Maykel ; Esteban, Patricia ; Martinez-Lostao, Luis (Universidad de Zaragoza) ; Pardo, Julián (Universidad de Zaragoza) ; Martínez de la Fuente, Jesús ; Galvez, Eva M.

Resumen: Gliotoxin (GT), a secondary metabolite produced by Aspergillus molds, has been proposed as a potential anti-tumor agent. Here we have developed a nanoparticle approach to enhance delivery of GT in tumor cells and establish a basis for its potential use as therapeutical drug. GT bound to magnetic nanoparticles (MNPs) retained a high anti-tumor activity, correlating with efficient intracellular delivery, which was increased in the presence of glucose. Our results show that the attachment of GT to MNPs by covalent bonding enhances intracellular GT delivery without affecting its biological activity. This finding represents the first step to use this potent anti-tumor agent in the treatment of cancer.

Idioma: Inglés

DOI: 10.3390/ma12071092

Año: 2019

Publicado en: Materials 12, 7 (2019), 1092 [17 pp]
ISSN: 1996-1944

Factor impacto JCR: 3.057 (2019)

Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 132 / 314 = 0.42 (2019) - Q2 - T2

Factor impacto SCIMAGO: 0.647 - Materials Science (miscellaneous) (Q2) - Condensed Matter Physics (Q2)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/FJCI-2017-31629
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Inmunología (Dpto. Microb.Med.Pr.,Sal.Públ.)