000079667 001__ 79667 000079667 005__ 20191126134633.0 000079667 0247_ $$2doi$$a10.1021/acs.bioconjchem.8b00245 000079667 0248_ $$2sideral$$a106448 000079667 037__ $$aART-2018-106448 000079667 041__ $$aeng 000079667 100__ $$aGarcés, Víctor 000079667 245__ $$aBacteria-carried iron oxide nanoparticles for treatment of anemia 000079667 260__ $$c2018 000079667 5060_ $$aAccess copy available to the general public$$fUnrestricted 000079667 5203_ $$aThe efficiency of maghemite nanoparticles for the treatment of anemia was sensibly higher when nanoparticles were incorporated onto the probiotic bacterium Lactobacillus fermentum (MNP-bacteria) than when administrated as uncoated nanoparticles (MNP). Plasma iron and hemoglobin, intestine expression of divalent metal transporter 1 (DMT1) and duodenal Cytochrome b (DcytB), as well as hepatic expression of the hormone hepcidin were fully restored to healthy levels after administration of MNP-bacteria but not of MNP. A magnetic study on biodistribution and biodegradation showed accumulation of maghemite nanoparticles in intestine lumen when MNP-bacteria were administrated. In contrast, MNP barely reached intestine. In vivo MRI studies suggested the internalization of MNP-bacteria into enterocytes, which did not occur with MNP. Transmission electronic microscopy confirmed this internalization. The collective analysis of results point out that L. fermentum is an excellent carrier to overcome the stomach medium and drive maghemite nanoparticles to intestine, where iron absorption occurs. Due the probiotic ability to adhere to the gut wall, MNP-bacteria internalize into the enterocyte, where maghemite nanoparticles are delivered, providing an adequate iron level into enterocyte. This paper advances a new route for effective iron absorption in the treatment of anemia. 000079667 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-67995-C3-3-R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64538-R$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512 000079667 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000079667 590__ $$a4.349$$b2018 000079667 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b9 / 78 = 0.115$$c2018$$dQ1$$eT1 000079667 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b70 / 294 = 0.238$$c2018$$dQ1$$eT1 000079667 591__ $$aCHEMISTRY, ORGANIC$$b9 / 57 = 0.158$$c2018$$dQ1$$eT1 000079667 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b44 / 172 = 0.256$$c2018$$dQ2$$eT1 000079667 592__ $$a1.551$$b2018 000079667 593__ $$aBioengineering$$c2018$$dQ1 000079667 593__ $$aBiomedical Engineering$$c2018$$dQ1 000079667 593__ $$aPharmacology$$c2018$$dQ1 000079667 593__ $$aOrganic Chemistry$$c2018$$dQ1 000079667 593__ $$aPharmaceutical Science$$c2018$$dQ1 000079667 593__ $$aBiotechnology$$c2018$$dQ1 000079667 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000079667 700__ $$aRodríguez-Nogales, Alba 000079667 700__ $$aGonzález, Ana 000079667 700__ $$aGálvez, Natividad 000079667 700__ $$aRodríguez-Cabezas, M. Elena. 000079667 700__ $$aGarcía-Martin, María L. 000079667 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, Lucía$$uUniversidad de Zaragoza 000079667 700__ $$aRondón, Deyanira 000079667 700__ $$aOlivares, Mónica 000079667 700__ $$aGálvez, Julio 000079667 700__ $$aDominguez-Vera, José M. 000079667 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000079667 773__ $$g29, 5 (2018), 1785-1791$$pBioconjug. chem.$$tBioconjugate Chemistry$$x1043-1802 000079667 8564_ $$s583726$$uhttps://zaguan.unizar.es/record/79667/files/texto_completo.pdf$$yPostprint 000079667 8564_ $$s73009$$uhttps://zaguan.unizar.es/record/79667/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000079667 909CO $$ooai:zaguan.unizar.es:79667$$particulos$$pdriver 000079667 951__ $$a2019-11-26-13:42:32 000079667 980__ $$aARTICLE