000079667 001__ 79667
000079667 005__ 20191126134633.0
000079667 0247_ $$2doi$$a10.1021/acs.bioconjchem.8b00245
000079667 0248_ $$2sideral$$a106448
000079667 037__ $$aART-2018-106448
000079667 041__ $$aeng
000079667 100__ $$aGarcés, Víctor
000079667 245__ $$aBacteria-carried iron oxide nanoparticles for treatment of anemia
000079667 260__ $$c2018
000079667 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079667 5203_ $$aThe efficiency of maghemite nanoparticles for the treatment of anemia was sensibly higher when nanoparticles were incorporated onto the probiotic bacterium Lactobacillus fermentum (MNP-bacteria) than when administrated as uncoated nanoparticles (MNP). Plasma iron and hemoglobin, intestine expression of divalent metal transporter 1 (DMT1) and duodenal Cytochrome b (DcytB), as well as hepatic expression of the hormone hepcidin were fully restored to healthy levels after administration of MNP-bacteria but not of MNP. A magnetic study on biodistribution and biodegradation showed accumulation of maghemite nanoparticles in intestine lumen when MNP-bacteria were administrated. In contrast, MNP barely reached intestine. In vivo MRI studies suggested the internalization of MNP-bacteria into enterocytes, which did not occur with MNP. Transmission electronic microscopy confirmed this internalization. The collective analysis of results point out that L. fermentum is an excellent carrier to overcome the stomach medium and drive maghemite nanoparticles to intestine, where iron absorption occurs. Due the probiotic ability to adhere to the gut wall, MNP-bacteria internalize into the enterocyte, where maghemite nanoparticles are delivered, providing an adequate iron level into enterocyte. This paper advances a new route for effective iron absorption in the treatment of anemia.
000079667 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-67995-C3-3-R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64538-R$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512
000079667 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000079667 590__ $$a4.349$$b2018
000079667 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b9 / 78 = 0.115$$c2018$$dQ1$$eT1
000079667 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b70 / 294 = 0.238$$c2018$$dQ1$$eT1
000079667 591__ $$aCHEMISTRY, ORGANIC$$b9 / 57 = 0.158$$c2018$$dQ1$$eT1
000079667 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b44 / 172 = 0.256$$c2018$$dQ2$$eT1
000079667 592__ $$a1.551$$b2018
000079667 593__ $$aBioengineering$$c2018$$dQ1
000079667 593__ $$aBiomedical Engineering$$c2018$$dQ1
000079667 593__ $$aPharmacology$$c2018$$dQ1
000079667 593__ $$aOrganic Chemistry$$c2018$$dQ1
000079667 593__ $$aPharmaceutical Science$$c2018$$dQ1
000079667 593__ $$aBiotechnology$$c2018$$dQ1
000079667 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000079667 700__ $$aRodríguez-Nogales, Alba
000079667 700__ $$aGonzález, Ana
000079667 700__ $$aGálvez, Natividad
000079667 700__ $$aRodríguez-Cabezas, M. Elena.
000079667 700__ $$aGarcía-Martin, María L.
000079667 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, Lucía$$uUniversidad de Zaragoza
000079667 700__ $$aRondón, Deyanira
000079667 700__ $$aOlivares, Mónica
000079667 700__ $$aGálvez, Julio
000079667 700__ $$aDominguez-Vera, José M.
000079667 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000079667 773__ $$g29, 5 (2018), 1785-1791$$pBioconjug. chem.$$tBioconjugate Chemistry$$x1043-1802
000079667 8564_ $$s583726$$uhttps://zaguan.unizar.es/record/79667/files/texto_completo.pdf$$yPostprint
000079667 8564_ $$s73009$$uhttps://zaguan.unizar.es/record/79667/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000079667 909CO $$ooai:zaguan.unizar.es:79667$$particulos$$pdriver
000079667 951__ $$a2019-11-26-13:42:32
000079667 980__ $$aARTICLE