Assessment of the effect of nitrogen concentration on fermentation and selection of a highly competitive Saccharomyces cerevisiae strain for efficient ethanol production

Portero Barahona, Patricia ; Martín Gil, Jesús ; Martín Ramos, Pablo (Universidad de Zaragoza) ; Briones Pérez, Ana ; Carvajal Barriga, Enrique Javier

Resumen: The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L−1, attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production.

Idioma: Inglés

DOI: 10.3390/en12132614

Año: 2019

Publicado en: Energies 12, 13 (2019), 2614 [12 pp.]
ISSN: 1996-1073

Factor impacto JCR: 2.702 (2019)

Categ. JCR: ENERGY & FUELS rank: 63 / 112 = 0.562 (2019) - Q3 - T2

Factor impacto SCIMAGO: 0.635 - Control and Optimization (Q2) - Electrical and Electronic Engineering (Q2) - Renewable Energy, Sustainability and the Environment (Q2) - Energy Engineering and Power Technology (Q2) - Fuel Technology (Q2) - Energy (miscellaneous) (Q2)

Tipo y forma: Article (Published version)

Área (Departamento): Area Ingeniería Agroforestal (Dpto. CC.Agrar.y Medio Natural)