Dual Role of Magnetic Nanoparticles as Intracellular Hotspots and Extracellular Matrix Disruptors Triggered by Magnetic Hyperthermia in 3D Cell Culture Models

Beola, L. ; Asín, L. (Universidad de Zaragoza) ; Fratila, R.M. ; Herrero, V. ; De La Fuente, J.M. (Universidad de Zaragoza) ; Grazú, V. (Universidad de Zaragoza) ; Gutiérrez, L. (Universidad de Zaragoza)

Resumen: Magnetic hyperthermia is a promising therapy for the localized treatment of cancer based on the exposure of magnetic nanoparticles to an external alternating magnetic field. In order to evaluate some of the mechanisms involved in the cellular damage caused by this treatment, two different 3D cell culture models were prepared using collagen, which is the most abundant protein of the extracellular matrix. The same amount of nanoparticles was added to cells either before or after their incorporation into the 3D structure. Therefore, in one model, particles were located only inside cells (In model), while the other one had particles both inside and outside cells (In&Out; model). In the In&Out; model, the hyperthermia treatment facilitated the migration of the particles from the outer areas of the 3D structure to the inner parts, achieving a faster homogeneous distribution throughout the whole structure and allowing the particles to gain access to the inner cells. The cell death mechanism activated by the magnetic hyperthermia treatment was different in both models. Necrosis was observed in the In model and apoptosis in the In&Out; model 24 h after the hyperthermia application. This was clearly correlated with the amount of nanoparticles located inside the cells. Thus, the combination of both 3D models allowed us to demonstrate two different roles of the magnetic particles during the hyperthermia treatment: (i) The modulation of the cell death mechanism depending on the amount of intracellular particles and (ii) the disruption of the collagen matrix caused by the extracellular nanoparticles.

Idioma: Inglés

DOI: 10.1021/acsami.8b18270

Año: 2018

Publicado en: ACS Applied Materials & Interfaces 10, 51 (2018), 44301-44313
ISSN: 1944-8244

Factor impacto JCR: 8.456 (2018)

Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 16 / 94 = 0.17 (2018) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 27 / 293 = 0.092 (2018) - Q1 - T1

Factor impacto SCIMAGO: 2.596 - Materials Science (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/COST-Action/TD1402-Radiomag
Financiación: info:eu-repo/grantAgreement/ES/DGA/FSE
Financiación: info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R
Financiación: info:eu-repo/grantAgreement/ES/UZ/UZ2018-CIE-03

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)