Estudio multicéntrico de cepas clínicas de SARM sensibles a antibióticos no-ß-lactámicos: líneas genéticas y producción de la leucocidina de Panton-Valentine

Ceballos, S. ; Aspiroz, C. ; Ruiz-Ripa, L. ; Azcona-Gutierrez, J.M. ; López-Cerero, L. ; López-Calleja, A.I. ; Álvarez, L. ; Gomáriz, M. ; Fernández, M. ; Torres, C. ; Ezpeleta, C. ; Martín, C. ; Arribas, J. ; Navarro, C. ; Arias, A. ; Fortuño, B. ; Pereira, J. ; Milagro, A. ; Torres, L. ; Soria-Blanco, L.M. ; Canut, A. ; Cordón, M.L. ; Megías, G. ; Calvo, J. ; Rezusta, A. (Universidad de Zaragoza)

Resumen: Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is considered a major cause of healthcare-associated (HA) and community-acquired (CA) infections. Considering non-ß-lactam susceptibility as a potential marker for mecC-MRSA and CA-MRSA, the aim of this study was to determine the frequency and the associated genetic lineages of non-beta-lactam-antibiotic susceptible MRSA (NBLS-MRSA) strains in a multicenter study in Spain.
Methods: A collection of 45 NBLS-MRSA strains recovered in the period from January to June 2016 from 12 Spanish hospitals was analyzed. Molecular typing through spa-type characterization, agr group and multi-locus-sequence typing was performed. Methicillin-resistant genes (mecA and mecC) as well as immune evasion cluster (scn-chp-sak-sea-sep, considering scn gene as the marker of IEC system) and Panton-Valentine leukocidin (PVL) genes were determined with PCR/sequencing.
Results: The NBLS-MRSA phenotype was uncommon in the 12 hospitals analyzed (NBLS-MRSA/MRSA frequency: 0.3%-7.7%). All strains contained the mecA gene (and none contained mecC). Twenty-two different spa-types were detected among NBLS-MRSA strains, with spa-t008/agr-I the most prevalent (27%). The main clonal complexes were (CC/%): CC8/42.2%, CC5/33.3% and CC30/4.4%, with ST8 and ST5 as the main sequence types. The PVL toxin was present in 38% of strains (with spa-types t008, t024, t019, t044, t068, t318 and t3060). The IEC genes were detected in 78% of strains: IEC type-B (n = 17), type-F (n = 16), type-A (n = 1) and type-E (n = 1); 10 MRSA isolates were scn-negative.
Conclusion: The NBLS-MRSA phenotype is uncommon in the analyzed hospitals; although no mecC-positive strains were detected, it could be a good marker for MRSA PVL-positive isolates (38%), frequently associated with CA-MRSA infections.

Idioma: Español

DOI: 10.1016/j.eimc.2019.01.015

Año: 2019

Publicado en: Enfermedades Infecciosas y Microbiologia Clinica 37, 8 (2019), 509-513
ISSN: 0213-005X

Factor impacto JCR: 1.654 (2019)

Categ. JCR: MICROBIOLOGY rank: 112 / 134 = 0.836 (2019) - Q4 - T3
Categ. JCR: INFECTIOUS DISEASES rank: 79 / 93 = 0.849 (2019) - Q4 - T3

Factor impacto SCIMAGO: 0.317 - Microbiology (medical) (Q3)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76571-R

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)