000089754 001__ 89754
000089754 005__ 20210902121712.0
000089754 0247_ $$2doi$$a10.3390/ijms21082701
000089754 0248_ $$2sideral$$a117854
000089754 037__ $$aART-2020-117854
000089754 041__ $$aeng
000089754 100__ $$aFernández-alegre, Estela
000089754 245__ $$aMelatonin non-linearly modulates bull spermatozoa motility and physiology in capacitating and non-capacitating conditions
000089754 260__ $$c2020
000089754 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089754 5203_ $$aBull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects mediated by melatonin receptors (pM), intracellular targets (nM–µM) or antioxidant activity (µM). Treatment effects were assessed as motility changes by computer-assisted sperm analysis (CASA) of motility and physiological changes by flow cytometry. Melatonin effects were more evident in capacitating conditions, with 100 pM reducing motility and velocity (VCL) while increasing a “slow” subpopulation. All concentrations decreased apoptotic spermatozoa and stimulated mitochondrial activity in viable spermatozoa, with 100 pM–1 µM increasing acrosomal damage, 10 nM–1 µM increasing intracellular calcium and 1 pM reducing the response to a calcium-ionophore challenge. In non-capacitating media, 1 µM increased hyperactivation-related variables and decreased apoptotic spermatozoa; 100 pM–1 µM increased membrane disorders (related to capacitation); all concentrations decreased mitochondrial ROS production. Melatonin concentrations had a modal effect on bull spermatozoa, suggesting a capacitation-modulating role and protective effect at physiological concentrations (pM). Some effects may be of practical use, considering artificial reproductive techniques.
000089754 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P
000089754 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000089754 590__ $$a5.923$$b2020
000089754 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 297 = 0.226$$c2020$$dQ1$$eT1
000089754 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b49 / 178 = 0.275$$c2020$$dQ2$$eT1
000089754 592__ $$a1.455$$b2020
000089754 593__ $$aCatalysis$$c2020$$dQ1
000089754 593__ $$aComputer Science Applications$$c2020$$dQ1
000089754 593__ $$aInorganic Chemistry$$c2020$$dQ1
000089754 593__ $$aSpectroscopy$$c2020$$dQ1
000089754 593__ $$aMolecular Biology$$c2020$$dQ1
000089754 593__ $$aOrganic Chemistry$$c2020$$dQ1
000089754 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1
000089754 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000089754 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000089754 700__ $$aÁlvarez-fernández, Indira
000089754 700__ $$aDomínguez, Juan Carlos
000089754 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000089754 700__ $$aMartínez-pastor, Felipe
000089754 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000089754 773__ $$g21, 8 (2020), 2701 [20 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000089754 8564_ $$s1724387$$uhttps://zaguan.unizar.es/record/89754/files/texto_completo.pdf$$yVersión publicada
000089754 8564_ $$s507970$$uhttps://zaguan.unizar.es/record/89754/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000089754 909CO $$ooai:zaguan.unizar.es:89754$$particulos$$pdriver
000089754 951__ $$a2021-09-02-09:21:48
000089754 980__ $$aARTICLE