000094572 001__ 94572 000094572 005__ 20231006143252.0 000094572 0248_ $$2sideral$$a105667 000094572 037__ $$aART-2017-105667 000094572 041__ $$aeng 000094572 100__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, D. 000094572 245__ $$aCirculating Exosomal Mir21 And Mir320 In Obstructive Sleep Apnea 000094572 260__ $$c2017 000094572 5060_ $$aAccess copy available to the general public$$fUnrestricted 000094572 5203_ $$aRational. Epidemiological studies indicate that there may be an association between obstructive sleep apnea (OSA) and cardiovascular and metabolic diseases. Some pro-inflammatory miRs (miR-21, miR320) critical for the immune response or hypoxia are often overexpressed in cancers and atherosclerosis. Aim. To examine the expression of miR-21& miR320 in circulating exosomes from patients with OSA. Methods: From a Sleep Unit and in the frame of a long-term longitudinal cohort study we selected 65 non-smokers OSA patients (apnea-hypopnea index -AHI- 30 events/ti) and 26 age, gender and BMI-matched controls (AHI < 5). All participants were free of comorbidities other than OSA at baseline (GovTrials, NCT014575421). At recruitment and yearly, carotid ultrasound was performed and subclinical atherosclerosis (SA) was defined as by the presence of carotid plaques of by an intima-media thickness > 0.85 mm. Plasma-derived exosomes were isolated by precipitation using miRCURY, , , Exosome Isolation Kit. Exosomes were characterized by transmission electron microscopy, dynamic light scattering assay and Western Blot analysis using CD63 and HSP70. Exosome total RNA was obtained using miRCURY"* RNA isolation kit. miR-21 -5p and miR-320-3p were analysed by real time quantitative PCR (RT-qPCR) using miRCURY LNA~ technology... 000094572 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI12-02175$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-1940 000094572 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000094572 590__ $$a15.239$$b2017 000094572 591__ $$aRESPIRATORY SYSTEM$$b2 / 59 = 0.034$$c2017$$dQ1$$eT1 000094572 591__ $$aCRITICAL CARE MEDICINE$$b2 / 33 = 0.061$$c2017$$dQ1$$eT1 000094572 592__ $$a5.942$$b2017 000094572 593__ $$aPulmonary and Respiratory Medicine$$c2017$$dQ1 000094572 593__ $$aCritical Care and Intensive Care Medicine$$c2017$$dQ1 000094572 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion 000094572 700__ $$0(orcid)0000-0001-6016-4726$$aMartin-Burrie, I.$$uUniversidad de Zaragoza 000094572 700__ $$aGil, V. 000094572 700__ $$0(orcid)0000-0001-9096-2294$$aMarin, J.M.$$uUniversidad de Zaragoza 000094572 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000094572 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina 000094572 773__ $$g195 (2017), A4523$$pAm. j. respir. crit. care med.$$tAmerican Journal of Respiratory and Critical Care Medicine$$x1073-449X 000094572 85641 $$uhttps://www.atsjournals.org/doi/abs/10.1164/ajrccm-conference.2017.195.1_MeetingAbstracts.A4523$$zTexto completo de la revista 000094572 8564_ $$s18174$$uhttps://zaguan.unizar.es/record/94572/files/texto_completo.pdf$$yVersión publicada 000094572 8564_ $$s426862$$uhttps://zaguan.unizar.es/record/94572/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000094572 909CO $$ooai:zaguan.unizar.es:94572$$particulos$$pdriver 000094572 951__ $$a2023-10-06-14:06:01 000094572 980__ $$aARTICLE