Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration

Smaida, R. ; Pijnenburg, L. ; Irusta, Silvia (Universidad de Zaragoza) ; Himawan, E. ; Mendoza, G. (Universidad de Zaragoza) ; Harmouch, E. ; Idoux-Gillet, Y. ; Kuchler-Bopp, S. ; Benkirane-Jessel, N. ; Hua, G.

Resumen: The treatment of osteochondral defects remains a challenge. Four scaffolds were produced using Food and Drug Administration (FDA)-approved polymers to investigate their therapeutic potential for the regeneration of the osteochondral unit. Polycaprolactone (PCL) and poly(vinyl-pyrrolidone) (PVP) scaffolds were made by electrohydrodynamic techniques. Hydroxyapatite (HAp) and/or sodium hyaluronate (HA) can be then loaded to PCL nanofibers and/or PVP particles. The purpose of adding hydroxyapatite and sodium hyaluronate into PCL/PVP scaffolds is to increase the regenerative ability for subchondral bone and joint cartilage, respectively. Humanbone marrow-derived mesenchymal stem cells (hBM-MSCs) were seeded on these biomaterials. The biocompatibility of these biomaterials in vitro and in vivo, as well as their potential to support MSC differentiation under specific chondrogenic or osteogenic conditions, were evaluated. We show here that hBM-MSCs could proliferate and differentiate both in vitro and in vivo on these biomaterials. In addition, the PCL-HAp could effectively increase the mineralization and induce the differentiation of MSCs into osteoblasts in an osteogenic condition. These results indicate that PCL-HAp biomaterials combined with MSCs could be a beneficial candidate for subchondral bone regeneration.

Idioma: Inglés

DOI: 10.3390/ma13143087

Año: 2020

Publicado en: MATERIALS 13, 14 (2020), 3087 [16 pp.]
ISSN: 1996-1944

Factor impacto JCR: 3.623 (2020)

Categ. JCR: METALLURGY & METALLURGICAL ENGINEERING rank: 17 / 80 = 0.213 (2020) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 152 / 333 = 0.456 (2020) - Q2 - T2
Categ. JCR: PHYSICS, CONDENSED MATTER rank: 27 / 69 = 0.391 (2020) - Q2 - T2
Categ. JCR: PHYSICS, APPLIED rank: 51 / 160 = 0.319 (2020) - Q2 - T1
Categ. JCR: CHEMISTRY, PHYSICAL rank: 79 / 162 = 0.488 (2020) - Q2 - T2

Factor impacto SCIMAGO: 0.682 - Materials Science (miscellaneous) (Q2) - Condensed Matter Physics (Q2)

Financiación: info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092

Tipo y forma: Article (Published version)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)