Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly

Baumann, Kevin N. ; Piantanida, Luca ; García-Nafría, Javier (Universidad de Zaragoza) ; Sobota, Diana ; Voïtchovsky, Kislon ; Knowles, Tuomas P.J. ; Hernández-Ainsa, Silvia (Universidad de Zaragoza)

Resumen: The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of the protein building blocks and clathrin self-assemblies to coat liposomes with biomaterials, advanced hybrid carriers can be derived. Here, we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently connecting DNA-based triskelion structures on the liposome surface inspired by the assembly of the protein clathrin. Dynamic light scattering, ¿-potential, confocal microscopy, and cryo-electron microscopy measurements independently demonstrate successful DNA coating. Nanomechanical measurements conducted with atomic force microscopy show that the DNA coating enhances the mechanical stability of the liposomes relative to uncoated ones. Furthermore, we provide the possibility to reverse the coating process by triggering the disassembly of the DNA coats through a toehold-mediated displacement reaction. Our results describe a straightforward, versatile, and reversible approach for coating and stabilizing lipid vesicles through the assembly of rationally designed DNA structures. This method has potential for further development toward the ordered arrangement of tailored functionalities on the surface of liposomes and for applications as hybrid nanocarriers.

Idioma: Inglés

DOI: 10.1021/acsnano.9b09453

Año: 2020

Publicado en: ACS nano 14, 2 (2020), 2316-2323
ISSN: 1936-0851

Factor impacto JCR: 15.881 (2020)

Categ. JCR: CHEMISTRY, PHYSICAL rank: 12 / 162 = 0.074 (2020) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 11 / 106 = 0.104 (2020) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 14 / 178 = 0.079 (2020) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 21 / 333 = 0.063 (2020) - Q1 - T1

Factor impacto SCIMAGO: 5.554 - Engineering (miscellaneous) (Q1) - Physics and Astronomy (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E47-17R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/RTI2018-095629-J-I00
Financiación: info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-CIE-04
Financiación: info:eu-repo/grantAgreement/ES/UZ/UZ2018-CIE-04

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)