000095537 001__ 95537
000095537 005__ 20211216131132.0
000095537 0247_ $$2doi$$a10.1016/j.talanta.2019.120392
000095537 0248_ $$2sideral$$a114709
000095537 037__ $$aART-2020-114709
000095537 041__ $$aeng
000095537 100__ $$aNavarro, Jesús
000095537 245__ $$aAnalytical possibilities of Putrescine and Cadaverine enzymatic colorimetric determination in tuna based on diamine oxidase: A critical study of the use of ABTS
000095537 260__ $$c2020
000095537 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095537 5203_ $$aThe joint determination of putrescine (Put) and cadaverine (Cad) in the presence of other biogenic amines is studied using their enzymatic reaction with diamine oxidase (DAO). Three alternative methods are studied based on the intrinsic colorimetric properties of DAO or horseradish peroxidase (HRP), and the use of 2, 2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) colorimetric reagent, respectively. In this last case an in-depth study is carried out in order to explain and solve some drawbacks usually associated with the use of this reagent (especially interferences, interaction with enzymes and instability), and to propose new analytical methodologies which this reagent allows to achieve (transient signal and the use of the violet species). Finally, the method has been applied to Put + Cad determination in a tuna sample without interference of other biogenic amines. The result has been compared with that obtained using a method based on HPLC-MS, which has allowed the new methodology to be validated.
000095537 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-17R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R
000095537 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000095537 590__ $$a6.057$$b2020
000095537 591__ $$aCHEMISTRY, ANALYTICAL$$b12 / 83 = 0.145$$c2020$$dQ1$$eT1
000095537 592__ $$a1.181$$b2020
000095537 593__ $$aAnalytical Chemistry$$c2020$$dQ1
000095537 593__ $$aSpectroscopy$$c2020$$dQ1
000095537 593__ $$aBiochemistry$$c2020$$dQ1
000095537 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000095537 700__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, Isabel$$uUniversidad de Zaragoza
000095537 700__ $$aLozano, Rebeca
000095537 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, Susana$$uUniversidad de Zaragoza
000095537 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000095537 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000095537 773__ $$g208 (2020), 120392 1-8$$pTalanta$$tTALANTA$$x0039-9140
000095537 85641 $$uhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85072765676&doi=10.1016%2fj.talanta.2019.120392&partnerID=40&md5=8d8955d8084cb7042c6d523fcb0329b0$$zTexto completo de la revista
000095537 8564_ $$s1222880$$uhttps://zaguan.unizar.es/record/95537/files/texto_completo.pdf$$yPostprint
000095537 8564_ $$s448342$$uhttps://zaguan.unizar.es/record/95537/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
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000095537 951__ $$a2021-12-16-13:02:14
000095537 980__ $$aARTICLE