Analytical possibilities of Putrescine and Cadaverine enzymatic colorimetric determination in tuna based on diamine oxidase: A critical study of the use of ABTS

Navarro, Jesús ; Sanz-Vicente, Isabel (Universidad de Zaragoza) ; Lozano, Rebeca ; de Marcos, Susana (Universidad de Zaragoza) ; Galbán, Javier (Universidad de Zaragoza)

Resumen: The joint determination of putrescine (Put) and cadaverine (Cad) in the presence of other biogenic amines is studied using their enzymatic reaction with diamine oxidase (DAO). Three alternative methods are studied based on the intrinsic colorimetric properties of DAO or horseradish peroxidase (HRP), and the use of 2, 2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) colorimetric reagent, respectively. In this last case an in-depth study is carried out in order to explain and solve some drawbacks usually associated with the use of this reagent (especially interferences, interaction with enzymes and instability), and to propose new analytical methodologies which this reagent allows to achieve (transient signal and the use of the violet species). Finally, the method has been applied to Put + Cad determination in a tuna sample without interference of other biogenic amines. The result has been compared with that obtained using a method based on HPLC-MS, which has allowed the new methodology to be validated.

Idioma: Inglés

DOI: 10.1016/j.talanta.2019.120392

Año: 2020

Publicado en: TALANTA 208 (2020), 120392 1-8
ISSN: 0039-9140

Originalmente disponible en: Texto completo de la revista

Factor impacto JCR: 6.057 (2020)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 12 / 83 = 0.145 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.181 - Analytical Chemistry (Q1) - Spectroscopy (Q1) - Biochemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-17R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)