000096185 001__ 96185
000096185 005__ 20210902121845.0
000096185 0247_ $$2doi$$a10.1186/s12951-020-00701-7
000096185 0248_ $$2sideral$$a120745
000096185 037__ $$aART-2020-120745
000096185 041__ $$aeng
000096185 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, M.$$uUniversidad de Zaragoza
000096185 245__ $$aIsolation of exosomes from whole blood by a new microfluidic device: proof of concept application in the diagnosis and monitoring of pancreatic cancer
000096185 260__ $$c2020
000096185 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096185 5203_ $$aBackground: Exosomes are endocytic-extracellular vesicles with a diameter around 100 nm that play an essential role on the communication between cells. In fact, they have been proposed as candidates for the diagnosis and the monitoring of different pathologies (such as Parkinson, Alzheimer, diabetes, cardiac damage, infection diseases or cancer). Results: In this study, magnetic nanoparticles (Fe3O4NPs) were successfully functionalized with an exosome-binding antibody (anti-CD9) to mediate the magnetic capture in a microdevice. This was carried out under flow in a 1.6 mm (outer diameter) microchannel whose wall was in contact with a set of NdFeB permanent magnets, giving a high magnetic field across the channel diameter that allowed exosome separation with a high yield. To show the usefulness of the method, the direct capture of exosomes from whole blood of patients with pancreatic cancer (PC) was performed, as a proof of concept. The captured exosomes were then subjected to analysis of CA19-9, a protein often used to monitor PC patients. Conclusions: Here, we describe a new microfluidic device and the procedure for the isolation of exosomes from whole blood, without any need of previous isolation steps, thereby facilitating translation to the clinic. The results show that, for the cases analyzed, the evaluation of CA19-9 in exosomes was highly sensitive, compared to serum samples.
000096185 536__ $$9info:eu-repo/grantAgreement/ES/UZ-DGA/T57-17R-P$$9info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715$$9info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE$$9info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092$$9info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007
000096185 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096185 590__ $$a10.435$$b2020
000096185 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b19 / 106 = 0.179$$c2020$$dQ1$$eT1
000096185 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b8 / 159 = 0.05$$c2020$$dQ1$$eT1
000096185 592__ $$a1.629$$b2020
000096185 593__ $$aApplied Microbiology and Biotechnology$$c2020$$dQ1
000096185 593__ $$aBioengineering$$c2020$$dQ1
000096185 593__ $$aBiomedical Engineering$$c2020$$dQ1
000096185 593__ $$aPharmaceutical Science$$c2020$$dQ1
000096185 593__ $$aMolecular Medicine$$c2020$$dQ1
000096185 593__ $$aNanoscience and Nanotechnology$$c2020$$dQ1
000096185 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000096185 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096185 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, V.$$uUniversidad de Zaragoza
000096185 700__ $$0(orcid)0000-0002-7742-9329$$aSesé, J.$$uUniversidad de Zaragoza
000096185 700__ $$0(orcid)0000-0002-8026-7391$$aPazo-Cid, R.$$uUniversidad de Zaragoza
000096185 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, G.$$uUniversidad de Zaragoza
000096185 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000096185 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, P.
000096185 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, J.$$uUniversidad de Zaragoza
000096185 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000096185 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000096185 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000096185 773__ $$g18 (2020), 150 [15 pp]$$pJ. nanobiotechnol.$$tJournal of Nanobiotechnology$$x1477-3155
000096185 8564_ $$s3844051$$uhttps://zaguan.unizar.es/record/96185/files/texto_completo.pdf$$yVersión publicada
000096185 8564_ $$s36837$$uhttps://zaguan.unizar.es/record/96185/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000096185 909CO $$ooai:zaguan.unizar.es:96185$$particulos$$pdriver
000096185 951__ $$a2021-09-02-10:26:41
000096185 980__ $$aARTICLE