Isolation of exosomes from whole blood by a new microfluidic device: proof of concept application in the diagnosis and monitoring of pancreatic cancer

Sancho-Albero, M. (Universidad de Zaragoza) ; Sebastián, V. (Universidad de Zaragoza) ; Sesé, J. (Universidad de Zaragoza) ; Pazo-Cid, R. (Universidad de Zaragoza) ; Mendoza, G. (Universidad de Zaragoza) ; Arruebo, M. (Universidad de Zaragoza) ; Martín-Duque, P. ; Santamaría, J. (Universidad de Zaragoza)

Resumen: Background: Exosomes are endocytic-extracellular vesicles with a diameter around 100 nm that play an essential role on the communication between cells. In fact, they have been proposed as candidates for the diagnosis and the monitoring of different pathologies (such as Parkinson, Alzheimer, diabetes, cardiac damage, infection diseases or cancer). Results: In this study, magnetic nanoparticles (Fe3O4NPs) were successfully functionalized with an exosome-binding antibody (anti-CD9) to mediate the magnetic capture in a microdevice. This was carried out under flow in a 1.6 mm (outer diameter) microchannel whose wall was in contact with a set of NdFeB permanent magnets, giving a high magnetic field across the channel diameter that allowed exosome separation with a high yield. To show the usefulness of the method, the direct capture of exosomes from whole blood of patients with pancreatic cancer (PC) was performed, as a proof of concept. The captured exosomes were then subjected to analysis of CA19-9, a protein often used to monitor PC patients. Conclusions: Here, we describe a new microfluidic device and the procedure for the isolation of exosomes from whole blood, without any need of previous isolation steps, thereby facilitating translation to the clinic. The results show that, for the cases analyzed, the evaluation of CA19-9 in exosomes was highly sensitive, compared to serum samples.

Idioma: Inglés

DOI: 10.1186/s12951-020-00701-7

Año: 2020

Publicado en: Journal of Nanobiotechnology 18 (2020), 150 [15 pp]
ISSN: 1477-3155

Factor impacto JCR: 10.435 (2020)

Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 19 / 106 = 0.179 (2020) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 8 / 159 = 0.05 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.629 - Applied Microbiology and Biotechnology (Q1) - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmaceutical Science (Q1) - Molecular Medicine (Q1) - Nanoscience and Nanotechnology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/UZ-DGA/T57-17R-P
Financiación: info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715
Financiación: info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE
Financiación: info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)
Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)