000097134 001__ 97134
000097134 005__ 20230323131625.0
000097134 0247_ $$2doi$$a10.1038/s41598-020-77076-0
000097134 0248_ $$2sideral$$a121110
000097134 037__ $$aART-2020-121110
000097134 041__ $$aeng
000097134 100__ $$0(orcid)0000-0001-5348-924X$$aOliván-Viguera, A.
000097134 245__ $$aMinimally invasive system to reliably characterize ventricular electrophysiology from living donors
000097134 260__ $$c2020
000097134 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097134 5203_ $$aCardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratome-sliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verified viability. Optically mapped transmembrane potentials confirmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and ß-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.
000097134 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/PID2019-105674RB-I00$$9info:eu-repo/grantAgreement/EUR/ERC-2014-StG-638284$$9info:eu-repo/grantAgreement/ES/DGA-FSE/T39-20R-BSICoS group$$9info:eu-repo/grantAgreement/ES/DGA-FSE/LMP124-18
000097134 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097134 590__ $$a4.379$$b2020
000097134 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 73 = 0.233$$c2020$$dQ1$$eT1
000097134 592__ $$a1.24$$b2020
000097134 593__ $$aMultidisciplinary$$c2020$$dQ1
000097134 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097134 700__ $$0(orcid)0000-0002-3194-7796$$aPérez-Zabalza, M.$$uUniversidad de Zaragoza
000097134 700__ $$0(orcid)0000-0002-2954-1068$$aGarcía-Mendívil, L.$$uUniversidad de Zaragoza
000097134 700__ $$0(orcid)0000-0003-2946-3044$$aMountris, K.A.
000097134 700__ $$aOrós-Rodrigo, S.
000097134 700__ $$0(orcid)0000-0002-9218-6062$$aRamos-Marquès, E.$$uUniversidad de Zaragoza
000097134 700__ $$aVallejo-Gil, J.M.
000097134 700__ $$aFresneda-Roldán, P.C.
000097134 700__ $$aFañanás-Mastral, J.
000097134 700__ $$aVázquez-Sancho, M.
000097134 700__ $$0(orcid)0000-0001-7482-2582$$aMatamala-Adell, M.$$uUniversidad de Zaragoza
000097134 700__ $$aSorribas-Berjón, F.
000097134 700__ $$aBellido-Morales, J.A.
000097134 700__ $$aMancebón-Sierra, F.J.
000097134 700__ $$aVaca-Núñez, A.S.
000097134 700__ $$0(orcid)0000-0002-2225-308X$$aBallester-Cuenca, C.$$uUniversidad de Zaragoza
000097134 700__ $$aMarigil, M.Á.
000097134 700__ $$aPastor, C.$$uUniversidad de Zaragoza
000097134 700__ $$0(orcid)0000-0003-3982-1263$$aOrdovás, L.
000097134 700__ $$0(orcid)0000-0002-0833-9717$$aKöhler, R.
000097134 700__ $$aDiez, E.
000097134 700__ $$0(orcid)0000-0002-1960-407X$$aPueyo, E.$$uUniversidad de Zaragoza
000097134 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000097134 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000097134 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000097134 773__ $$g10, 1 (2020), 19941 [13 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000097134 8564_ $$s699721$$uhttps://zaguan.unizar.es/record/97134/files/texto_completo.pdf$$yVersión publicada
000097134 8564_ $$s310054$$uhttps://zaguan.unizar.es/record/97134/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097134 909CO $$ooai:zaguan.unizar.es:97134$$particulos$$pdriver
000097134 951__ $$a2023-03-23-12:57:37
000097134 980__ $$aARTICLE