Surface modification and characterization of membrane for platelet-derived growth factor (PDGF) purification in blood

Rosales, Abigail
Bacchin, Patrice (dir.)

Universidad de Zaragoza, CIEN, 2020

Máster Universitario Erasmus Mundus en Ingeniería de Membranas

Resumen: The extraction and the purification of platelet-derived growth factor (PDGF) from blood could have a potential in diabetic wound care. The current procedure involves multiple steps and a simpler separation process could improve its productivity. This report is related to a Ph.D. program (MOSAIC 3D project). One of the objectives is to modify PVDF membrane with a copolymer that could selectively capture platelets. The copolymer should avoid unwanted interactions with other blood proteins or it can be conjugated with a blood protein and induce platelet capture. This study intends to optimize the coating procedure using polystyrene-block-poly(acrylic acid) copolymer and investigate its interaction with major blood proteins – albumin, ɣ-globulin, and fibrinogen. Concentration, coating time, and operating process were varied. Coating distribution and static protein adsorption were studied using FTIR mapping. 5mg/mL copolymer concentration, 2h coating time, and drying the coating before washing are the optimum coating conditions. All three proteins have a similar amount of adsorption with the pristine and coated membranes which is attributed to the short PAA chains (only 10wt.% of the copolymer). The pure water permeability decline of the coated membranes at 1 and 5mg/mL copolymer concentration is minimal (6.15 and 11.8% respectively). The surface remained coated after water filtration. It is recommended to 1) test other copolymers or use PS-b-PAA with longer PAA chains, 2) investigate competitive protein adsorption, and 3) conduct filtration tests with protein solution to identify the selectivity of the membrane towards each protein and evaluate fouling.

Tipo de Trabajo Académico: Trabajo Fin de Master